Genetic diversity analysis of Triplophysa brevicauda in the upper reaches of Lancang River in Yunnan Province
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Q319.1

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    Abstract:

    For further realizing the genetic diversity and provide experimental data of germplasm resources for Triplophysa brevicauda in the upper reaches of Lancang River in Yunnan Province, 77 individuals including 4 geographic populations (Biaocun, Yezhi, Lidi and Wu Nonglong) were analyzed. After sequencing, the total length of 921 bp of D-loop control region were obtained, and 22 variable sites (including 8 singleton variable sites and 14 parsimony informative sites) and 21 haplotypes were identified in whole populations, respectively; the haplotypic diversity (Hd) and nucleotide diversity (π) of Triplophysa brevicauda in the upper reaches of Lancang River was 0.837-0.942 and 0.005 16-0.005 72, respectively; among them, the highest Hd was identified in Lidi population, the lowest Hd was identified in Biaocun population, and a genetic character of "high Hd low π" was presented; The results of AMOVA indicated that most of genetic variation (97.91%) came from within population and little of genetic variation (2.09%) was came from between populations; no significant genetic differentiation was detected between the comparison groups (Fst=-0.021 3, P=0.805); the farthest genetic distance (0.005 67) of Triplophysa brevicauda was identified between Biaocun and Lidi population, and the closest genetic distance (0.005 11) was found between Yezhi and Wu Nonglong population; no recent population expansion event happened in the evolutionary history of Triplophysa brevicauda, which was proved by the evidence of non-poisson distribution of nucleotide mismatch and insignificant neutral test result (Tajima's D=0.624, P=0.737;Fu's Fs=-0.669, P=0.377). The results showed relatively abundant genetic diversity and low level of genetic differentiationo of Triplophysa brevicauda in the upper reaches of Lancang River.

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赵静霞,熊合勇,吴俊颉,金方彭,高海涛,赵承远,雷春云,李光华.云南澜沧江上游短尾高原鳅遗传多样性分析[J].上海海洋大学学报,2022,31(1):52-60.
ZHAO Jingxia, XIONG Heyong, WU Junjie, JIN Fangpeng, GAO Haitao, ZHAO Chengyuan, LEI Chunyun, LI Guanghua. Genetic diversity analysis of Triplophysa brevicauda in the upper reaches of Lancang River in Yunnan Province[J]. Journal of Shanghai Ocean University,2022,31(1):52-60.

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History
  • Received:December 02,2020
  • Revised:February 01,2021
  • Adopted:May 27,2021
  • Online: January 13,2022
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