Molecular identification of Tribolodon “silver beachhead” migratory population in Suifen River

Abstract:

The taxonomic status of “silver beachhead” in Tribolodon migratory population in Suifen River has always been controversial. In this study, we used DNA barcoding COⅠ and gene markers(fst and mitfa) to identify 115 individuals from Suifen River, including Tribolodon hakonensis (40),T. brandtii(38) and “silver beachhead”(34), and 3 individuals of T. hakonensis from Lake Osorezan, Japan. The results of mitochondrial COⅠgene amplification showed that a total of 11 haplotypes were obtained,of which 5 haplotypes were exclusive to T. hakonensis, 5 haplotypes were exclusive to T. brandtii, and 1 haplotype (hap6) was exclusively shared by Japanese population of T. hakonensis,“silver beachhead” population shared 1 haplotype with T. hakonensis, and shared 3 haplotypes with T. brandtii.Cluster analysis did not support the “silver beachhead” population as an independent species.The results of amplification of two gene markers showed that “silver beachhead” population had both genotypes of T. hakonensis and T. brandtii.Combining the above two analysis results,it is found that there are hybrid individuals of T. hakonensis and T. brandtiiin “silver beachhead”.In conclusion, this study confirmed the taxonomic status of the “silver beachhead” population, that is, “silver beachhead” is a mixed population composed of T. hakonensis, T. brandtii and their hybrids.The results of this study provide a scientific basis for the classification, conservation of germplasm resources and wild release and enhancement of Tribolodon.