Research on expression of virus in Cyprinid herpesvirus 3 (CyHV-3) F4 disease resistant strains

Abstract:

The new variety was bred to F₄,and the disease resistance traits have reached stability. F₄ was used as the experimental material,and TK and ORF72 were used as virus marker genes to analyze the expression of F₄,a resistant strain of Cyprinus herpesvirus (CyHV-3),in different stages of viral infection, to investigate the effect of F₄ on disease resistance and its virus expression pattern from the perspective of virus expression.The results showed that: The expression trends of spleen and kidney tissues of the two genes at different infection stages were consistent,with an upward trend from 24 h to 144 h and a downward trend from 144 h to 288 h.The spleen and kidney of the unselected group and the kidney of the selected group were significantly different at 144 h compared with other periods. The expression of both genes in spleen tissue was lower than that in kidney tissue.The two genes in the breeding group and the non-breeding group were significantly different between 96 h and 144 h,and the two genes in the breeding group were significantly different at 96 h and 144 h in the ORF72,and the other periods were significantly different. The expression levels of two genes in the breeding group were lower than those in the non-breeding group.The two genes in spleen tissue were significantly different from 96 h to 168 h,and the ORF72 was also significantly different at 192 h and 216 h;the two genes in kidney tissue were significantly different from 96 h to 168 h;ORF72 was also significantly different at 192 h.The above results show that the disease resistance of the selective group is stronger than that of the non-selected group,in which the kidney tissue has better disease resistance than the spleen,and the immune resistance of the selected kidney tissue is greatly improved.At present, no selective breeding of other fisher has reached a stable stage in disease resistance.This study can provide important guidance for other fishes in the study of viral expression.