Combination of acidic electrolyzed water with ultrasonic for killing Vibrio parahaemolyticus

Abstract:

This study evaluated the bactericidal effects were evaluated against Vibrio parahaemolyticus by acidic electrolyzed water (AEW) combined with ultrasound which is a method different from the traditional sterilization methods. The effects were compared by viable plate count analysis. The physiological response was studied by scanning electronic microscopy (SEM) and cell membrane permeability revealed by protein leakage. Flow cytometry analysis revealed changes in the physiological states of V.parahaemolyticus. The effects of the combination of SAEW and ultrasound on the reductions of V.parahaemolyticus were evaluated in comparison with acidic electrolyzed water or ultrasound. The results revealed that AEW combined with ultrasonic showed bacterial reduction in V.parahaemolyticus by 2.09 log CFU/mL and the sub-lethal bacterial reduction was 1.80 log CFU/mL,when compared to the reduction of 0.63 log CFU/mL and the sub-lethal bacterial reduction of 0.05 log CFU/mL(P<0.05) after ultrasonic treatment only. Scanning electron microscopy (SEM) results showed that electrolyzed water combined with ultrasonic treatment had obvious destructive effect on the cell structure of V.parahaemolyticus. The protein leakage of V.parahaemolyticus detected by bicinchoninic acid (BCA) were 226.596 μg/mL, respectively, and difference was significant (P<0.05). Flow cytometry analysis showed that the cells became smaller and the change in particle size increased. In summary, acidic electrolyzed water combined with ultrasound could be more effective in reducing V.parahaemolyticus, the physiological response changes, protein leakage and changes in the physiological states, when compared with acidic electrolyzed water or ultrasound alone. The combination of acidic electrolyzed water and ultrasound could be an new technique to reduce the risk of V.parahaemolyticus.