Using Miseq sequencing to analyze seasonal soil microbial community dynamics in reclaimed Scirpus mariqueter coastal wetlands

Abstract:

By Miseq high-throughput sequencing technology, soil microbial community dynamics and the associated soil physiochemical characteristics were studied. The reclaimed Scirpus mariqueter wetland in Shanghai Nanhui Dongtan coastal area was the study site, which is just undergoing large scale wetlands reclamation impacts. Results showed the dense area and the light beach of S. mariqueter soil microbial communities were significantly different (P<0.001). The dense area of S. mariqueter is higher than the light beach area. The seasonal variation of microbial diversity and abundance in the same area was significant (P<0.001), and the spring and summer microbial diversity and abundance was lower than that in the autumn and winter. Proteobacteria, Bacteroidetes, Acidobacteria, Chloroflexi, Planctomycetes, Actinobacteria, Cyanobacteria, Gemmatimonadetes, and Nitrospirae were relatively abundant, and the seasonal dominant microbial community was different. Redundancy analysis showed that microbial community structure was significantly correlated with factors including soil salinity, dry wet ratio and nitrate nitrogen. This study is helpful to understand the micromechanism during S. mariqueter wetlands spatial sprawling and promote the fast remediation of S. mariqueter wetlands.