Abstract:Pinghu oil and gas field submarine pipeline has ruptured in 2012 near Daishan, but related toxic test of Pinghu crude oil is less. In order to investigate the toxic effect of Pinghu crude oil and its water-accommodated fractions(WAF), the acute toxicity effects of Pinghu crude oil and its WAF on Mugil cephalus, Palaemon carincauda, Sinonovacula constricta and Ruditapes philippinarum were studied,respectively. The results showed that both of Pinghu crude oil and its WAF had significant dose-effect relationship on tested marine organisms. The 96 h LC50 values of Pinghu crude oil for Mugil cephalus, Palaemon carincauda, Sinonovacula constricta, Ruditapes philippinarum were 865 mg/L, 343 mg/L, 2 441 mg/L and 5 221 mg/L, respectively. The 96 h LC50 values of WAF for Mugil cephalus, Palaemon carincauda, Sinonovacula constricta, Ruditapes philippinarum were 1.697 mg/L,0.645 mg/L, 4.535 mg/L, 5.783 mg/L, respectively. The order of the 96h LC50 for four test organisms did not change between Pinghu crude oil and its WAF, but WAF was more toxic than crude oil.