Analysis of microsatellite polymorphism and its application in parentage determination of Sinonovacula constricta

Abstract:

The Microsatellite-enhanced genomic library of Sinonovacula constricta was constructed using magnetic beads enrichment method. A total of 18 pairs of microsatellite primers were developed and screened out, of which 8 pairs showed high levels of genetic polymorphism. With these 8 pairs, the parentage determination was estimated and evaluated. The result revealed that, for the 18 pairs of microsatellite primers in 12 individuals, the number of alleles ranged from 2 to 6 and the average was 3, the observed and expected heterozygosis varies from 0 to 1.000, and from 0.290 to 0.779, respectively, and the polymorphism information content (PIC) changed from 0.239 to 0.703. The single parent exclusion rate(E-1P)was 0.583 to 0.916 if parents were both unknown, while that was 0.406 to 0.830 when only one of the parental genotype was known. Meanwhile, it was identified that with the increasing number of offspring, the exclusion rate of these markers still remains in a higher range. Thus, these pairs can be applied as microsatellite markers for identifying progeny genetic relationship, which is beneficial and significant for Sinonovacula constricta standardized cultivation and pedigree breeding.