Effects of acute handling stress on serum biochemical parameters and HSP70 gene expression in Alosa sapidissima broodstocks

Abstract:

This experiment studied the effect of acute handling stress on serum biochemical parameters and HSP70mRNA level in gill and liver of Alosa sapidissima broodstocks. The air exposure was applied in the acute handling stress of Alosa sapidissima broodstocks. This experiment set one stressless control group and three different intensity stress groups, separately exposed air 15 s, 30 s and 60 s. Serum cortisol(COR), triiodothyronine(T3), thyroxine(T4), glucose(GLU), triglyceride (TG), total cholesterol (TC), total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione S-transferase (GST), lysozyme (LZM), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), Na⁺, K⁺, Cl^-, Ca²⁺ concentrations and gill, liver HSP70 mRNA level were determined after sampling. The results showed that compared with the control group, the treatment group had the following serum biochemical parameters significantly increased: COR, ALT, AST, GST, LZM, SOD, MDA, GLU, TG, TC, TP, K⁺ and Ca²⁺ (P<0.05). CAT and T4 decreased significantly(P<0.05) after air exposure, while T3, Na⁺ and Cl^- had no significant difference(P>0.05). The changes in serum biochemical parameters indicate that the liver and heart of the Alosa sapidissima damage index significantly increased, suggesting that liver and heart may have been damaged to a certain extent. Additionally, compared with the control group, there was no significant change in gill HSP70 mRNA level in the experimental group (P>0.05). Liver HSP70 mRNA level in 15s, 30s and 60s were significantly higher in the exposed group (P<0.05). Acute handling stress can improve the level of HSP70 mRNA in liver.