Hepatoprotective effects of Lycium barbarum polysaccharides on carbon tetrachloride induced primary hepatocyte injury in Jian Carp

Abstract:

In order to study the hepatoprotective effects of Chinese herbal medicine Lycium barbarum poly-saccharide(LBP)against carbon tetrachloride-induced hepatocyte injury in Cyprinus carpio var.jian.Different concentrations of LBP(0.1 mg/mL, 0.2 mg/mL, 0.4 mg/mL) were added to the carp primary hepatocytes before(pre-treatment),after(post-treatment)and both before and after(pre-and post-treatment)incubation of the hepatocytes with CCl₄.After 12 h, the cell and supernatant were collected, and then the levels of glutamatepyruvate transaminase (GPT),glutamate oxalate transaminase(GOT), lactate dehydrogenase(LDH),malondialdehyde(MDA), superoxide dismutase(SOD), cell viability,tumor necrosis factor-α(TNF-α),Interleukin-1β(IL-1β),immunoglobulin M(IgM)and cytochrome p450 2E1(CYP2E1)were measured.The results showed that CCl₄ at 8 mmol/L in the culture medium significantly elevated the levels of GPT, GOT, LDH, MDA, TNF-α, IL-1β, IgM and CYP2E1, and significantly reduced levels of SOD and cell viability; however, treatment with LBP attenuated the adverse changes of these biochemical indexes, especially in pre-treatment and pre-and post-treatment; the changed levels of GPT, GOT, LDH, MDA,TNF-α, IL-1β, IgM and CYP2E1and the elevated levels of SOD and cell viability were significantly inhibited in a dose-dependent manner, while the post-treatment of hepatocytes with LBP only significantly reduced the levels of LDH,TNF-α and CYP2E1.Based on the results, it is indicated that LBP may have the hepatoprotective effects against carbon tetrachloride-induced hepatocyte injury in Cyprinus carpio var.jian and may be used as a hepatoprotective agent in fish liver diseases.