Parentage identification of Heilongjiang wild carp and German mirror carp cross combinations on microsatellite DNA markers
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    Abstract:

    A set of 8 microsatellite markers were used for parentage identification in obverse cross group of Heilongjiang carp♀×German mirror carp, and inverse cross group of German mirror carp♀×Heilongjiang carp. The results showed that: (1) observed heterozygosity values of parents and offspring were 0.607 1, 0.714 3, 0.681 8 and 0.708 3, 0.538 6, 0.743 8, and polymorphism information content were 0.442 1, 0.281 3, 0.553 5 and 0.673 5, 0.375 9, 0.624 1 in two groups, indicating that all 8 microsatellite loci showed polymorphism. (2) In the obverse cross group, the accumulative exclusion rate using 8 microsatellite loci was 99.92% and the appraisal ability was 98.00% while confidence was 95%. In the inverse cross group, the accumulative exclusion rate was 99.99% and the appraisal ability was 99.00%. (3) about relation between microsatellite markers and appraisal ability, we compared the accumulative exclusion rates and the appraisal ability using 3, 4, 5, 6, 7, 8 microsatellite markers. 6 and 8 high polymorphism markers were successfully applied in paternity determination of two cross groups.

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葛会争,贾智英,李池陶,柏盈盈,武 耀,石连玉.黑龙江野鲤与德国镜鲤杂交组合的亲子鉴定分析[J].上海海洋大学学报,2012,21(4):489-494.
GE Hui-zheng, JIA Zhi-ying, LI Chi-tao, BAI Ying-ying, WU Yao, SHI Lian-yu. Parentage identification of Heilongjiang wild carp and German mirror carp cross combinations on microsatellite DNA markers[J]. Journal of Shanghai Ocean University,2012,21(4):489-494.

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  • Online: July 18,2012
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