Studies on lactate dehydrogenase isozymes and DNA content in different tissues of Coilia nasus

Abstract:

The aim of this study was to provide technical parameters for the foundation of genetic background and resources in Coilia nasus.In the present study, lactate dehydrogenase isozymes (LDH) in liver, eye, kidney, muscle, gill and serum, and DNA content in tail fin, gill, muscle, gonad and liver were studied by isozyme and flow cytometry techniques. The results indicated that: (1) The LDH in different tissues had a tissue specialty. All the tissues exhibited five isozyme bands and same relative migration rate, except for LDH c expression. No LDH2 bands were found in muscle and serum. (2) No significant differences were observed in DNA content in gill, muscle and liver （P＞0.05）, but DNA content in those tissues was significantly lower than that in the tail fin and ovary cells （P＜0.05）. The ratio of DNA content in gill, muscle and liver and haploid spermatide were 2.16,2.19 and 2.22, respectively. Moreover, the hepatocytes which were easily isolated from tissue and made into single cell suspension were good materials for ploidy analysis by flow cytometry.