Quantitative trait loci analysis for activity of acid phosphatase and alkaline phosphatase in mirror carp

Abstract:

A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolysing phosphoric acid monoesters, which is widely distributed in most organisms and expressed in lots of tissues. This phosphatase is necessary enzyme for growth, maintaining metabolic homeostasis and keeping the body healthy. In this research, a whole genome scan was conducted on 190 progenies from a fullsib family using 992 microsatellite markers and interval mapping strategy for identifying QTL of activity of acid phosphatase and alkaline phosphatase traits in intestinal tissue. The results indicated that: five QTL affected the activity of acid phosphatase, of which three QTL were at the 99% chromosomewide level located on the LG2（CA2049-CA2371），LG25（HLJ2941-HLJ3946） and LG28（CA2263-HLJ2873） and the remaining two QTL were at 95% chromosomewide level explaining 8.1%-38.9% of the total variation, and two QTL affecting the activity of alkaline phosphatase were identified at 95% chromosomewide level located at LG8 and LG13 explaining 7.3% and 7% of the total variation, respectively.