Expressions of Toll receptor and lysozyme mRNA among tissues of white shrimp,Litopenaeus vannamei

Abstract:

The relative expression levels of Toll receptor and lysozyme mRNA in different tissues (haemolymph, gill, muscle and hepatopancreas) of the white shrimp, Litopenaeus vannamei, were quantitatively detected with realtime PCR technology. The results indicated that the expressions of Toll receptor and lysozyme mRNA among tissues were significantly different. The relative expression of Toll receptor mRNA in haemolymph was significantly higher than that in gill as well as in muscle and hepatopancreas, and the relative expression of Toll receptor mRNA in gill was significantly higher than those in muscle and hepatopancreas. The relative expressions of lysozyme mRNA in haemolymph and in gill were significantly higher than those in muscle and hepatopancreas. While there was no significant difference between relative expressions of lysozyme mRNA in haemolypha and gill. There was no significant difference between the variation coefficients of Toll receptor and lysozyme mRNA expressions in haemolymph and in gill. It is therefore suggested that haemplymph and gill were the suitable material for assays of Toll receptor and lysozyme mRNA expression levels for L. vannamei.