Determination of vitellogenin mRNA expression in ovary and hepatopancreas of shrimp Fenneropenaeus chinensis

Abstract:

The vitellogenin mRNA gene was expressed in both ovary and hepatopancreas of shrimp Fenneropenaeus chinensis. In this paper, the expression of vitellogenin mRNA in the ovary and hepatopancreas at different ovarian development stages, including multiplication oogoniium, previtellogenesis, primary vitellogenesis, secondary vitellogenesis and declining stages was determined by realtime fluorescence quantitative PCR. The total RNA was extracted from the ovary and hepatopancreas and the target gene was amplified by realtime fluorescence quantitative PCR with special primers. The results show that in ovary, the expression of mRNA increased from multiplication oogoniium to primary vitellogenesis stage. The relative expression levels were 0.04，0.23 and 1.03 respectively, but decreased at secondary vitellogenesis stage to 0.16. However, in declining stage it was 1.00. In hepatopancreas, the expression of mRNA increased from multiplication oogoniium to secondary vitellogenesis stage. The relative expression levels were 0.01，0.07，0.19 and 1.29 respectively，and it decreased to 0.74 at decling stage.