Identification of pathogen from pet chelonian with ophthalmia and the clinic therapy

Abstract:

Symptoms of ophthalmia of pet chelonian included eye closed, eyelid got red and membranae nictitans moved. It is a common disease on pet chelonian. A dominant bacterial strain isolated from the sick chelonian’s eye is assigned as the strain A5. The artificial infection test proved that the isolated A5 was the pathogenic bacterium that caused the disease. Synthesizing the morphology, physiological and biochemical characterization, and 16S rDNA of A5 was sequenced, the results showed that strain A5 exhibited the highest levels of similarity to the Pseudomonas fluorescens bv.C. The drug sensitivity test of strain A5 revealed that the pathogen was sensitive to drugs such as ofloxacin, gentamicin, tenebrimycin and piperacillin. The forty ophthalmic pet chelonians were divided into 8 groups to be treated and the efficacy analyzed in different treatments. The results showed that the drug effect of ofloxacin was better than sulfacetamide sodium and chloramphenicol. The curative effect of sick chelonian treated at 25 ℃ was better than at 5 ℃, and doing surgery was good for the cure of ophthalmia. Adding the dose of drug and intramuscular injection had no significant difference. The study could be helpful for clinic diagnosis in pet chelonian disease.