study of the microorganism using biomolecular technology from the Yangtze Estuary based on the technology of 16S rRNA

Abstract:

This paper is mainly based on microorganism samples of seawater which were gathered around the Yangtze Estuary from Oct.2007 to June 2008. The marine microorganism species were identified by using amplifications of 16S rRNA gene with primer 27F and 1492R, cloning and sequencing techniques. Through the database of NCBI, using the software of Mega 4.0, molecular phylogenetic tree was established with the sequences of 16S rRNA. In this paper, microorganism samples of seawater were respectively gathered in winter and summer at the same destination. Seventeen genera including fiftythree strains were achieved from the analysis of the four samples. Microbial community structure changes with temperature obviously, and dominant species vary with temperature. In winter, the Acinetobacter was the dominant species, and the molecular phylogenetic tree reflects a few genetic differences; In summer, microbial community structure was more complex, in which the Shewanella and Pseudomonas were the dominant species, and the molecular phylogenetic tree reflected greater genetic differences; In the waters of different temperature, microbial species shows different advantages obviously. Molecular phylogenetic tree reflects the genetic differences with obvious seasonal variation distribution.