Analysis of genetic diversity among five populations of Cyprinus carpio by TRAP markers

Abstract:

Target Region Amplification Polymorphism (TRAP) technique was adopted to assess genetic diversity among five populations of common carp (Cyprinus carpio) including purse red carp, Huanghe carp, Jian carp, Xingguo red carp and Heilongjiang carp by using 10 pairs of primer with high polymorphism. The fixed primers were designed from the sequence of GHR gene. Out of the 168 bands amplified by 10 primer combinations, 134 (80.41%) were polymorphic. The average of polymorphism information content (PIC) was 0.29. The AMOVA analysis result revealed 96.97% variance came from individuals within population which indicated that great genetic variation existing within the populations. The fixation index (F_ST) for TRAP was estimated to be 0.03026 and showed the significant genetic differentiation among populations (P<0.05). The cluster analysis based on the TRAP marker polymorphism of the target gene indicated that Jian carp and Xingguo red carp fell in one cluster, Huanghe carp and Heilongjiang carp fell in another cluster, and finally they clustered with purse red carp.