Comparing the competitive indirect enzyme-linked immunosorbent assay based on monoclonal antibody and High Performance Liquid Chromatography methods to determinate the ciprofloxacin residues in fishery products

Abstract:

For sensitive and specifically detection of ciprofloxacin (CIP) residues in fishery products,this study is aimed at generating and developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) based on CIP-specific monoclonal antibodies (McAb). The sensibility,accuracy and repeatability of ci-ELISA method was compared to those of High Performance Liquid Chromatography(HPLC) method.By concentration and purification process,the limit of detection (LOD) of ci-ELISA and HPLC method could reach to 10 μg /kg and 1 μg /kg respectively.we detected the recovery rates were 79.28%-92.92% and 86.27%-97.32% while relative standard deviation (RSD) varying from 3.21%-8.88% and 2.66%-5.52% for these methods.Between the two methos,ci-ELISA method was cheaper and more efficient while HPLC method was more sensitive and stable.