SRAP analysis of genetic variation between female and male swimming crab (Portunus trituberculatus)

Abstract:

Using the SRAP-PCR optimization system for P.trituberculatus,this study applied sequence-related amplified polymorphism (SRAP) technique with 17 primer pairs screened out of 88 primer combinations to analyze the genetic variation of female and male populations.146 loci were obtained and the percentage of polymorphic loci was 69.78% in female population and 79.07% in male population.Nei’s gene diversity was 0.231 5 and 0.268 2,Shannon’s information index was 0.347 8 and 0.402 6.Genetic similarity index ranged from 0.595 9 to 0.841 7 and the genetic distance was 0.158 3 to 0.404 1 between female and male.A dendrogram was constructed using TFPGA.6 loci were identified from 5 primer combinations.Male genotype is dominant in 4 loci,whose ratio was 12.5% and 20%, and female ratio was 100% in dominant individuals of 2 loci.