Determination of trichlorfon and dichlorvos residues in fishery products by gas chromatography

Abstract:

Gas chromatography method was developed for determination of trichlorfon and dichlorvos residues in fishery products by comparing chromatography conditions and the sample pretreatment. The samples were extracted with acetone-water (2+1, v/v) and discolored by activated carbon, lipids were removed by ZnAC, then the residues were re-extracted by dichloromethane and determined by gas chromatography with flame photometric detector (GC-FPD) . The linear range of trichlorfon and dichlorvos were 0.1~20 μg/mL and 0.025~5 μg/mL (r²≥0.999 5) . When spiked with 0.04, 0.4, 4 mg/kg of trichlorfon and 0.01, 0.1, 1 mg/kg of dichlorvos, the average recoveries ranged from 74.8% to 106.8% and their relative standard deviations (RSD) were between 1.12% and 13.7%. The detection limits of trichlorfon and dichlorvos were 0.04 mg/kg and 0.01 mg/kg separately. This method is simple, reliable and can be applied to detection of trichlorfon and dichlorvos residues in fishery products.