Cloning and sequence analysis of serine protease gene of Aeromonas sobria strain TL97528 from Trionyx sinensis

Abstract:

Aeromonas sobria TL97528,isolated from soft-shelled turtle(Trionyx sinensis),is the pathogen of Trionyx sinensis bacterial disease.And serine protease is the major virulence factor of aeromonas.A pair of primers was designed according to the nucleotide sequences of the serine protease gene of aeromonas species reported in GenBank.Desired fragment about 809 bp was amplified from TL97528 genome by the specific primers.The PCR product was cloned into PMD 18-T vector and sequenced.The sequence has been deposite...