Fractional genomic libraries constructing and microsatellites DNA screening of Chinese mitten crab(Eriocheir sinensis)

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Fractional genomic libraries constructing and microsatellites DNA screening of Chinese mitten crab(Eriocheir sinensis)
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Affiliation:MAO Rui-xin1,2,LU Cui-yun1,LIU Fu-jun3,ZHAO Ying-ying1,GU Jing-jing3,CHANG Yu-mei1,LIANG Li-qun1,SUN Xiao-wen1(1.Heilongjiang River Fisheries Research Institute,Chinese Acadamy of Fishery Sciences,Harbin 150076,China,2.College of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,3.College of Life Science and Technology,Dalian Fisheries College,Dalian 116023,China)
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Abstract:

In this experiment,microsatellite DNAs were isolated from Chinese mitten crab(Eriocheir sinensis) genome by combining biotin capture method and radioactive labeling hybridization.The purified ligated-DNA was hybridized with biotin-labeled simple sequence repeats(SSR) probes(GA)12,and the mixture was incubated with streptavidin-coated magnetic beads.The selected single-strand microsatellite DNA was obtained by removing the SSR-uncombined fragments.After capture of target fragments by magnetic beads and PCR a...

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毛瑞鑫,鲁翠云,刘福军,赵莹莹,谷晶晶,常玉梅,梁利群,孙效文.中华绒螯蟹部分基因组文库构建和微卫星位点的筛选[J].上海海洋大学学报,2009,(2).
MAO Rui-xin, LU Cui-yun, LIU Fu-jun, ZHAO Ying-ying, GU Jing-jing, CHANG Yu-mei, LIANG Li-qun, SUN Xiao-wen. Fractional genomic libraries constructing and microsatellites DNA screening of Chinese mitten crab(Eriocheir sinensis)[J]. Journal of Shanghai Ocean University,2009,(2).

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