Histology structure of thymus and immunohistochemistry of antibody secreting cell in mandarin fish (Siniperca chuatsi)

Abstract:

The thymus of mandarin fish(Siniperca chuatsi)was studied by general histology method and immunohistochemical method.The thymus is located on the edge of the gill cover close to the opercular cavity,triangle,is distributed symmetrically.Lobules of thymus are more evident along with increasing age.The parenchyma of thymus is divided into outer zone,middle zone and inner zone,with middle zone and inner zone being equal to cortex and medulla of mammal thymus respectively.The outer zone chiefly consists of squamous epithelium cells and mucous cells.Large numbers of reticulate fibres and lympoid cells are shown in middle zone,and the inner zone is full of deep-blue staining lympoid cells which are distributed loosely.Furthermore,Hassal's corpuscules and epithelial cysts are observed under light microscope.The result of immunohistochemistry shows that B lympoid cells of thymus are mostly distribute in the outer zone and middle zone,especially between the two lobules.cell membrane is positive evidently,brownish yellow.