Homologue analysis of chemokine gene SCYA 107 between lctalurus punctatus and lctalurus furcatus

Abstract:

The positive BAC clones were identified through screening BAC DNA library of channel catfish(Ictalurus punctatus).Positive clone 006_I13 was partial sequenced using primer-walking stratagem,and 2557 bp-length DNA sequence containing SCYA 107 gene was analysed.SCYA 107 gene consisted of 4 exons and 3 introns.The sequence which spliced into 4 exons was completely same with the SCYA 107 cDNA sequence published before,which encodes 96 amino acids.It is typical one member of chemokine CC subfamily based on owning a CC and two separate cysteines at the N-terminal of amino acid.The upstream of SCYA 126 gene contained some immune-related transcription factor binding sites.There exists a big difference between SCYA 107 proteins of channel catfish and blue catfish.The SCYA 107 protein of channel catfish falls into the scope of mouse chemokine CCL 20,however,the SCYA 107 protein of blue catfish is much closer to human CCL 8.The big difference between the two catfishes results from an insertion with five-bp length of nucleotide ACAAA in channel catfish SCYA 107 gene,which leads to ORF shift.