RAPD analysis of second filial generation derived from boshi carp and frigid-resistance strain of red purse carp

Abstract:

Parentage identification of the second filial generation, which derived from Beshi carp and frigidresistance strain of red purse carp, was carried out with RAID techniques. Totally 5 out of 180 RAID primer with fine polymorphisms were screened to represent high polymorphisms. Statistical analysis of the polymorphisms showed that the RAPD bands in parents were totally found in their offspring. The bands of filial generations appeared 87.0%, 100.0%, 92.1%, 82.8% and 92.6% in parents from primers AB02, AB05, AC20, AI10 and AI13, respectively. It indicates that it is feasible to identify parents of the common carp with RAPD techniques.