Analyzing the polymorphisms of Takifugu rubripes with microsatellite

Abstract:

Two populations of Takifugu rubripes domesticated in different places were analyzed with the Takifugu frbripes microsatellite marker and the pattern of each sample was obtained.Altogether 20 primers were used in this experiment,of which 16 primers were assessed for genetic diversity for 10 individuals.The PCR products were electrophoresed by 2% agarose gel,and the data like allelic frequencies and analyzed by statistic method.The results indicated:mean number of alleles were 1.875 0 and 2.437 5,PIC value(polymorphism information content)were 0.275 1 and 0.379 4 and the observed heterozygosity values over all loci were 0.025 and 0.200.All of these indices indicated that genetic diversity of Takifugu rubripes was not inspiring.