Molecular cloning and sequence analysis of insulin-like growth factor I(IGF-I)cDNA from Cirrhinus molitorella
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S917

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    Abstract:

    Total RNA was isolated from Cirrhinus molitorella liver tissue. The cDNA encoding insulin like growth factor I (IGF I) was amplified by reverse transcription polymerase chain reaction (RT PCR) strategy using isolated total RNA as template.The amplified cDNA fragment was inserted to vector pUCm T. The cloned cDNA was sequenced and the amino acid sequence of Cirrhinus molitorella IGF I was predicted .The nucleotide sequence and deduced amino acid sequence shared 95.2% and 100% homology with that of Cyprinus carpio mature IGF I peptide. Analysis of E domain indicated that cloned Cirrhinus molitorella belonged to IGF I Ea 2 subtype.

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张殿昌,江世贵,苏天凤,蔡云川,许新萍.鲮胰岛素样生长因子Ⅰ(IGF-Ⅰ)cDNA的分子克隆和序列分析[J].上海海洋大学学报,2002,(2):97-101.
ZHANG Dian chang, JIANG Shi gui, SU Tian feng, CAI Yun chuan, XU Xin ping . Molecular cloning and sequence analysis of insulin-like growth factor I(IGF-I)cDNA from Cirrhinus molitorella[J]. Journal of Shanghai Ocean University,2002,(2):97-101.

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