Isolation and identification of pathogen of the cataract disease and its immunological control in Rana tigrina rugulosa

Abstract:

This paper reports the pathogen and the immunological protective method of the cataract disease in cultured Rana tigrina rugulosa in Hainan. The pathogen isolated from the ill frog with typical characteristics was identified as Flavobacterium meningitidis. In all the 20 kinds of antimicrobial agents tested in this experiment, only vancomycin is susceptible and cefoperazone is intermediate susceptible to the pathogen, as to the other 18 kinds of antimicrobics are unsusceptible. After inoculation the health frogs with the vaccine prepared from the formalin killed Flavobacterium meningitidis, their sera agglutination titer and resistance to cataract disease improved obviously. It shows that the cataract disease in cultured Rana tigrina rugulosa can be successfully prevented by the vaccine.