文章摘要
李学艳,王君文,易金玉,黄莹颖,张朝燕,吴文惠.含虾青素的雨生红球藻粉自微乳的制备及体外评价[J].上海海洋大学学报,2021,30(5):940-949
含虾青素的雨生红球藻粉自微乳的制备及体外评价
Preparation of Haematococcus pluvialis powder self-microemulsion containing astaxanthin and in vitro evaluation
投稿时间:2020-07-15  修订日期:2020-09-11
DOI:10.12024/jsou.20200703109
中文关键词: 虾青素  雨生红球藻粉  自微乳  伪三元相图  体外评价  响应面法
英文关键词: astaxanthin  Haematococcus pluvialis powder  self-microemulsion  pseudo ternary phase diagram  in vitro evaluation  response surface methodology
基金项目:国家自然科学基金(81750110548)
作者单位E-mail
李学艳 上海海洋大学 食品学院, 上海 201306  
王君文 上海海洋大学 食品学院, 上海 201306  
易金玉 上海海洋大学 食品学院, 上海 201306  
黄莹颖 上海海洋大学 食品学院, 上海 201306  
张朝燕 上海海洋大学 食品学院, 上海 201306
国家淡水水产品加工技术研发分中心, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业农村部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
chyzhang@shou.edu.cn 
吴文惠 上海海洋大学 食品学院, 上海 201306
国家淡水水产品加工技术研发分中心, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业农村部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
 
摘要点击次数: 521
全文下载次数: 276
中文摘要:
      为了提高雨生红球藻粉中虾青素的溶出度,使用自乳化给药系统。采用伪三元相图法筛选液体自乳化给药系统的最佳组成,使用未经提取的雨生红球藻粉制备含虾青素的自微乳,并进行体外评价。以油相、乳化剂、助乳化剂用量为影响因素,平均粒径、多分散指数(PDI)、雨生红球藻粉中虾青素的载药量为评价指标,响应面法优化处方。然后,考察自微乳外观形态、粒径、PDI、Zeta电位、载药量、溶出度。最佳处方为油相(三乙酸甘油酯)用量5%,乳化剂(吐温80)用量67.86%,助乳化剂(聚乙二醇800)用量27.14%,在透射电镜下观察到微乳呈圆球状分布,大小均匀,平均粒径(50.32±0.33)nm,PDI(0.131±0.02),Zeta电位(-14.6±0.06)mV,虾青素载药量为(3.06±0.23)mg/g。含虾青素的雨生红球藻粉自微乳制备简单,增加了虾青素在水中的溶解度,有利于胃肠道吸收,体系具有良好的稳定性,在食品和药品工业中具有广阔的应用前景。
英文摘要:
      In order to improve the dissolution of astaxanthin from Haematococcus pluvialis powder, a self-emulsifying drug delivery system was used. The pseudo ternary phase diagram method was used to screen the best composition of the liquid self-emulsifying drug delivery system, and the unextracted Haematococcus pluvialis powder was used to prepare astaxanthin-containing self-microemulsion, and to conduct in vitro evaluation. Taking oil phase, emulsifier, and co-emulsifier dosage as influencing factors, average particle size, polydispersity index (PDI), and drug loading of astaxanthin in Haematococcus pluvialis powder as evaluation indicators, response surface methodology was used to optimize the prescription. Then, the appearance, particle size, PDI, Zeta potential, drug loading, and dissolution of the self-microemulsion were investigated. The best prescription is oil phase (glyceryl triacetate) dosage of 5%, emulsifier (Tween 80) dosage of 67.86%, and co-emulsifier (polyethylene glycol 800) dosage of 27.14%. Under the transmission electron microscope, it was observed that the microemulsion was distributed in a spherical shape with uniform size. The average particle size was (50.32±0.33) nm, PDI (0.113±0.02), the Zeta potential was(-14.6±0.06) mV, and the astaxanthin drug loading was (3.06±0.23) mg/g. The self-microemulsion of Haematococcus pluvialis powder containing astaxanthin was simple to prepare, which increased the solubility of astaxanthin in water, and was conducive to gastrointestinal absorption. The system has good stability and has broad application prospects in food and drug industry.
HTML 查看全文   
关闭