圆紫菜快速生长新品系的分离与特性分析
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S968.431

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国家重点研发计划“海洋农业与淡水渔业科技创新”重点专项(2023YFD2400103,2023YFD2400102);国家自然科学基金青年基金(32102773);上海市青年科技英才扬帆计划(21YF1417100);上海海洋大学青年教师科研启动项目(A2-2006-23-200304)


Isolation and characterization of a new strain with fast growth in Pyropia suborbiculata (Bangiales, Rhodophyta)
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    摘要:

    与中国南方紫菜栽培物种坛紫菜(Pyropia haitanensis)相比,圆紫菜(Pyropia suborbiculata)具有更强的耐高温性,但其圆形藻体不适合生产栽培。为此研究者采用人工诱变技术,从圆紫菜野生型品系中分离出了数个长型突变体。本研究在此基础上,采用60Co-γ射线辐照圆紫菜长型突变体CCT-2的叶状体,以生长速度为主要选育指标,从其后代中筛选出了1个快速生长的新品系PS-M1。在23 ℃下培养60 d的叶状体,PS-M1的平均体长和长宽比分别为68.1 cm和26.3,分别是CCT-2的2.7和2.4倍。在23 ℃下培养30 d的叶状体再在30 ℃下培养30 d,PS-M1CCT-2的体长分别增加了6.4和3.0倍。在30 ℃下培养14 d,PS-M1CCT-2的壳孢子存活率分别为47.4%和42.2%,两者之间无显著性差异。在23 ℃下培养45 d的叶状体,PS-M1的叶绿素a和总藻胆蛋白含量分别为6.5和64.9 mg/g,分别比CCT-2增加了20.4%和22.9%;PS-M1的平均厚度为33.6 μm,比CCT-2薄了15.8%。连续10 d的壳孢子放散总量,PS-M1高达223.3万个/贝壳,是CCT-2的4.8倍。综上所述,相比圆紫菜长型突变体CCT-2,新品系PS-M1的叶状体生长速度更快,且在叶状体耐高温性和色素蛋白含量,以及壳孢子放散量等方面也表现出明显优势,有望被应用于中国南方沿海生产栽培。

    Abstract:

    Compared with Pyropia haitanensis cultivated in southern China, Pyropia suborbiculata has stronger high-temperature resistance, but its round algae are not suitable for production. Therefore, several long-type mutants were isolated from the wild-type strain of P. suborbiculata by artificial mutagenesis. In this study, the blades of the long-type mutant (CCT-2) of P. suborbiculata were induced by 60Co-γ ray, and a new strain (PS-M1) with fast growth was screened from its progeny with growth rate as the main breeding index. The PS-M1 blades cultured at 23 ℃ for 60 days had a mean length of 68.1 cm and a length-width ratio of 26.3, which were 2.7 and 2.4 times of CCT-2, respectively. The blades of PS-M1 and CCT-2 cultured at 23 ℃ for 30 days and then cultured at 30 ℃ for another 30 days, their mean length had increased by 6.4 and 3.0 times, respectively. The survival rates of conchospores of PS-M1 and CCT-2 were 47.4 and 42.2%, respectively, after 14 days of culture at 30 ℃, with no significant difference between them. The blades cultured at 23 ℃ for 45 days had a mean chlorophyll a content of 6.5 mg/g and phycobiliprotein content of 64.9 mg/g in PS-M1, which were 20.4% and 22.9% higher than those of CCT-2, respectively. The thickness of PS-M1 was 33.6 μm, which was 15.8% thinner than that of CCT-2. The total numbers of conchospores released by PS-M1 was up to 2.223 million per shell for 10 consecutive days, which was 4.8 times that of CCT-2. In conclusion, compared with long-type mutant (CCT-2), the new strain (PS-M1) of P. suborbiculata has a higher growth rate, better resistance to high-temperature and also shows obvious advantages in main pigments protein contents, and number of conchospores releasing, which has potential to be applied in commercial cultivation.

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应家轩,丁洪昌,严兴洪.圆紫菜快速生长新品系的分离与特性分析[J].上海海洋大学学报,2025,34(3):519-529.
YING Jiaxuan, DING Hongchang, YAN Xinghong. Isolation and characterization of a new strain with fast growth in Pyropia suborbiculata (Bangiales, Rhodophyta)[J]. Journal of Shanghai Ocean University,2025,34(3):519-529.

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  • 收稿日期:2024-04-02
  • 最后修改日期:2024-05-03
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  • 在线发布日期: 2025-05-23
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