环境胁迫对福寿螺肝胰脏抗氧化酶活性及丙二醛含量的影响

Effects of environmental stress on the activities of antioxidant enzymes and malondialdehyde content in hepatopancreas of apple snails

摘要:

为研究广州、上海、成都3地的福寿螺在不同温度、不同盐度、不同溶解氧环境胁迫下,肝胰脏超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量的变化情况,阐明环境胁迫对福寿螺生理的影响机理。结果表明：在温度、溶解氧、盐度单因子胁迫下,福寿螺肝胰脏的SOD活性、MDA含量均呈先上升后下降的变化趋势,且在24 ~48 h时达到最大值,在72 h时恢复到初始水平。在温度-盐度复合胁迫下,福寿螺肝胰脏SOD活性及MDA含量高于单因素胁迫,温度和盐度表现出协同作用。单因素或复合胁迫下,福寿螺肝胰脏SOD活性及MDA含量存在地区差异。

Abstract:

In this paper, the changes of superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in hepatopancreas of Pomacea canaliculate in Guangzhou, Shanghai and Chengdu under different temperature, salinity and dissolved oxygen environmental stresses were studied to clarify the mechanism of environmental stress on the physiology of Pomacea canaliculate. The experimental results showed that under the stress of temperature, dissolved oxygen and salinity, the SOD activity and MDA content of the hepatopancreas of the snails increased first and then decreased, and reached the maximum value at 24 h~48 h, and returned to the initial level at 72 h. Under the combined temperature-salinity stress, the SOD activity and MDA content in the hepatopancreas of the snails were higher than those under the single factor stress, and the temperature and salinity showed a synergistic effect. Under single or complex stress, SOD activity and MDA content in liver and pancreas of the snails varied from region to region.