牡蛎中细菌及其抗性基因多样性分析
CSTR:
作者:
中图分类号:

TS207.3;TS201.6

基金项目:

上海市科技兴农项目(沪农科创字〔2019〕第3-7号);上海市优秀学术带头人项目(21XD1401200);上海市教育委员会科研创新计划(2017-01-07-00-10-E00056)


Diversity analysis of bacteria and their resistance genes in oyster
Author:
  • XIE Qingchao

    XIE Qingchao

    College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China;Laboratory of Food Quality and Safety Testing, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Hao

    LI Hao

    College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Ruihong

    LI Ruihong

    Hohhot Agricultural and Animal Products Quality Safety Center, Hohhot 010020, Nei Mongol, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHANG Hongmin

    ZHANG Hongmin

    College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China;Laboratory of Food Quality and Safety Testing, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LIU Haiquan

    LIU Haiquan

    College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China;Hohhot Agricultural and Animal Products Quality Safety Center, Hohhot 010020, Nei Mongol, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • PAN Yingjie

    PAN Yingjie

    College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China;Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHAO Yong

    ZHAO Yong

    College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China;Laboratory of Food Quality and Safety Testing, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [32]
  • |
  • 相似文献
  • | | |
  • 文章评论
    摘要:

    本研究以中国四个沿海地区所产牡蛎为研究对象,分析了不同样品中细菌多样性以及肠道、腮和肌肉3种组织器官中抗生素及重金属抗性基因分布情况及分布规律,最后采用相关性分析比较了抗性基因之间的相关性。结果表明:微生物含量占比前5位的细菌分别为不可培养的拟杆菌、未分类的芽孢杆菌、未分类的假单胞菌、河流弧菌及嗜盐杆菌斯氏弓形菌,包含了2类腐败菌属和1类致病菌属;从抗性基因种类来看:氨基糖苷类ARGs检出率最高(84.1%),β-内酰胺类ARGs和喹诺酮类ARGs检出率最低(57.1%);以牡蛎不同组织器官分析抗性基因:肌肉中检出率(65.6%)>腮中检出率(59.1%)>肠道中检出率(58.4%);以不同样品来源分析抗性基因检出率:GXBH样品整体检出率最高(72.7%),SDWH1整体检出率最低(54.5%),不同来源样品在抗性基因种类及丰度上各样品差异不显著,在进行PCA分析后发现同一样品可以进行明显的归一化分类,说明同一样品不同组织器官中抗性基因关联性较大;从不同抗性基因ARGs和HTGs的相关性分析来看,CARBqnrS相关性最高,ChrR则与sulAmecA有较大相关性,CopA与各大类ARGs都有一定相关性,说明不同类型抗性基因有存在共用一个基因盒的可能性,从对不同来源样品的相关性分析来看,基本遵循了以不同产地样品进行归一化的分类,说明养殖环境可以对牡蛎中抗性基因的分布产生影响。通过以上研究,分析了牡蛎中微生物优势菌群,初步比较了抗性基因与品种、产地、各组织器官之间的相关性,为进一步研究抗性产生机制打下了基础。

    Abstract:

    As a filter feeding bivalve marine organism, oyster is easy to accumulate microorganisms due to its living habits. In this study, oysters from four coastal areas of China were selected as the research objects. The microbie diversity in different samples and the distribution of antibiotic and heavy metal resistance genes in intestinal, gill and muscle were analyzed. Finally, the correlation between resistance genes was compared by correlation analysis. The results showed that:(1) The top five bacteria with microbial content are uncultured Bacteroides, unclassified Bacillus cohn, unclassified Pseudomonas, Vibrio fluvialis and Arcobacter skirrowii, including two types of spoilage bacteria and one type of pathogenic bacteria; (2) In terms of resistance gene types, the detection rate of aminoglycoside args is the highest (84.1%), β-lactam args and quinolone args were the lowest (57.1%). Detected by different tissues and organs of oyster, the detection rate in muscle (65.6%) > gill (59.1%) > intestine (58.4%). Detected from different sample sources, the overall detection rate of GXBH samples is the highest (72.7%), and the overall detection rate of SDWH1 is the lowest (54.5%), and the samples from different sources have no significant difference in the type and abundance of resistance genes. After PCA analysis, it is found that the same sample can be significantly normalized and classified, indicating that the resistance genes in different tissues and organs of the same sample are highly correlated. (3) From the correlation analysis of different resistance genes ARGs and HTGs, CARB and qnrS have the highest correlation, chrR has a great correlation with sulA and mecA, and copA has a certain correlation with all kinds of ARGs, indicating that different types of resistance genes have the possibility of sharing a gene box. From the correlation analysis of samples from different sources, it basically follows the normalized classification of samples from different places of origin. It shows that the breeding environment can affect the distribution of resistance genes in oysters. Through the above studies, the dominant microbial groups in oysters were analyzed, and the correlation between resistance genes and varieties, places of origin, tissues and organs was preliminarily compared, which laid a foundation for further research on the mechanism of resistance.

    参考文献
    [1] 农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会. 中国渔业统计年鉴-2021[M]. 北京:中国农业出版社, 2021. Fishery Administration of the Ministry of Agriculture and Rural Areas, National Fisheries Technology Extension Center, China Society of Fisheries. 2021 China fisheries statistical yearbook[M]. Beijing:China Agricultural Press, 2021.
    [2] 国家贝类产业技术体系. 中国牡蛎产业发展报告[J]. 中国水产, 2021(6):20-31. National Shellfish Industry Technology System. China oyster industry development report[J]. China Fisheries, 2021(6):20-31.
    [3] YE L, LU Z, LI X H, et al. Antibiotic-resistant bacteria associated with retail aquaculture products from Guangzhou, China[J]. Journal of Food Protection, 2013, 76(2):295-301.
    [4] LEVY S B, MARSHALL B. Antibacterial resistance worldwide:causes, challenges and responses[J]. Nature Medicine, 2004, 10(12 S):S122-S129.
    [5] ALLEN H K, DONATO J, WANG H H, et al. Call of the wild:antibiotic resistance genes in natural environments[J]. Nature Reviews Microbiology, 2010, 8(4):251-259.
    [6] ZHU D, XIANG Q, YANG X R, et al. Trophic Transfer of antibiotic resistance genes in a soil detritus food chain[J]. Environmental Science & Technology, 2019, 53(13):7770-7781.
    [7] STEPANAUSKAS R, GLENN T C, JAGOE C H, et al. Coselection for microbial resistance to metals and antibiotics in freshwater microcosms[J]. Environmental Microbiology, 2006, 8(9):1510-1514.
    [8] JO S, SHIN C, SHIN Y, et al. Heavy metal and antibiotic co-resistance in Vibrio parahaemolyticus isolated from shellfish[J]. Marine Pollution Bulletin, 2020, 156:111246.
    [9] JIANG L, HU X L, XU T, et al. Prevalence of antibiotic resistance genes and their relationship with antibiotics in the Huangpu River and the drinking water sources, Shanghai, China[J]. Science of the Total Environment, 2013, 458-460:267-272.
    [10] OUOBA L I I, LEI V, JENSEN L B. Resistance of potential probiotic lactic acid bacteria and bifidobacteria of African and European origin to antimicrobials:determination and transferability of the resistance genes to other bacteria[J]. International Journal of Food Microbiology, 2008, 121(2):217-224.
    [11] HUANG S F, DAI W, SUN S, et al. Prevalence of plasmid-mediated quinolone resistance and aminoglycoside resistance determinants among carbapeneme non-susceptible Enterobacter cloacae[J]. PLoS One, 2012, 7(10):e47636.
    [12] XU J, XU Y, WANG H M, et al. Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river[J]. Chemosphere, 2015, 119:1379-1385.
    [13] MAYNARD C, FAIRBROTHER J M, BEKAL S, et al. Antimicrobial resistance genes in enterotoxigenic Escherichia coli O149:K91 isolates obtained over a 23-year period from pigs[J]. Antimicrobial Agents & Chemotherapy, 2003, 47(10):3214-3221.
    [14] BOUSKILL N J, BARNHART E P, GALLOWAY T S, et al. Quantification of changing Pseudomonas aeruginosa sodA, htpX and mt gene abundance in response to trace metal toxicity:a potential in situ biomarker of environmental health[J]. FEMS Microbiology Ecology, 2007, 60(2):276-286.
    [15] HE Y, JIN L L, SUN F J, et al. Antibiotic and heavy-metal resistance of Vibrio parahaemolyticus isolated from fresh shrimps in Shanghai fish markets, China[J]. Environmental Science and Pollution Research, 2016, 23(15):15033-15040.
    [16] 蓝蔚青, 王蒙, 陈梦玲, 等. 水产品特定腐败菌群体感应及其植物源抑制剂研究进展[J]. 食品与机械, 2018, 34(9):185-190. LAN W Q, WANG M, CHEN M L, et al. Research progress of specific spoilage organisms quorum sensing and plant-source inhibitors in aquatic products[J]. Food and Machinery, 2018, 34(9):185-190.
    [17] JIANG H, YU T, YANG Y T, et al. Co-occurrence of antibiotic and heavy metal resistance and sequence type diversity of Vibrio parahaemolyticus isolated from Penaeus vannamei at freshwater farms, seawater farms, and markets in Zhejiang province, China[J]. Frontiers in Microbiology, 2020, 11:1294.
    [18] 陈慧斌, 刘智禹, 陈绍军, 等. 基于PCR-DGGE技术的冷藏牡蛎鳃部菌群分析[J]. 西南大学学报(自然科学版), 2013, 35(4):151-156. CHEN H B, LIU Z Y, CHEN S J, et al. On characterization of gill associated bacterial flora in chilled stored oyster based on PCR-DGGE[J]. Journal of Southwest University (Natural Science Edition), 2013, 35(4):151-156.
    [19] 付娇娇, 彭织云, 刘海泉, 等. 酸性电解水处理后南美白对虾贮藏过程中肠道微生物的多样性变化[J]. 食品工业科技, 2015, 36(4):344-347, 356. FU J J, PENG Z Y, LIU H Q, et al. Changes of acidic electrolyzed water on intestinal microflora diversity of Penaeus vannawei during storage[J]. Science and Technology of Food Industry, 2015, 36(4):344-347, 356.
    [20] PRAPAIWONG N, WALLACE R K, ARIAS C R. Bacterial loads and microbial composition in high pressure treated oysters during storage[J]. International Journal of Food Microbiology, 2009, 131(2/3):145-150.
    [21] 沈安梅. 上海市南汇区2007年感染性腹泻病监测分析[J]. 上海预防医学杂志, 2008, 20(12):592-593. SHEN A M. Surveillance and analysis of infectious diarrhea in Nanhui District of Shanghai in 2007[J]. Shanghai Journal of Preventive Medicine, 2008, 20(12):592-593.
    [22] 励建荣. 海水鱼类腐败机制及其保鲜技术研究进展[J]. 中国食品学报, 2018, 18(5):1-12. LI J R. Research progress on spoilage mechanism and preservation technology of marine fish[J]. Journal of Chinese Institute of Food Science and Technology, 2018, 18(5):1-12.
    [23] HAN J E, KIM J H, CHERESCA C H JR, et al. First description of the qnrS-like (qnrS5) gene and analysis of quinolone resistance-determining regions in motile Aeromonas spp. from diseased fish and water[J]. Research in Microbiology, 2012, 163(1):73-79.
    [24] KIM M, KWON T H, JUNG S M, et al. Antibiotic resistance of bacteria isolated from the internal organs of edible snow crabs[J]. PLoS One, 2013, 8(8):e70887.
    [25] 娄阳, 张昭寰, 肖莉莉, 等. 食品源抗生素抗性基因的来源与分布状况研究进展[J]. 食品工业科技, 2015, 36(12):368-374. LOU Y, ZHANG Z H, XIAO L L, et al. Research progress of source and distribution of antibiotic resistance genes in food[J]. Science and Technology of Food Industry, 2015, 36(12):368-374.
    [26] 黄志坚, 陈旭凌, 路晓峰, 等. 水产养殖生物和养殖环境细菌鉴定及抗生素抗性基因检测[J]. 中山大学学报(自然科学版), 2012, 51(6):92-96. HUANG Z J, CHEN X L, LU X F, et al. Identification and antibiotic resistance genes detection of bacteria in aquaculture organisms and aquatic environment[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2012, 51(6):92-96.
    [27] 崔丁心, 陈玲, 陈慧, 等. 东寨港河口牡蛎中重金属-抗生素抗性细菌分析[J]. 海南师范大学学报(自然科学版), 2016, 29(3):263-267, 273. CUI D X, CHEN L, CHEN H, et al. Analysis of resistant bacteria of antibiotic and heavy metal in oysters from Dongzhaigang estuary[J]. Journal of Hainan Normal University (Natural Science), 2016, 29(3):263-267, 273.
    [28] D'COSTA V M, KING C E, KALAN L, et al. Antibiotic resistance is ancient[J]. Nature, 2011, 477(7365):457-461.
    [29] DAANE L L, MOLINA J, SADOWSKY M J. Plasmid transfer between spatially separated donor and recipient bacteria in earthworm-containing soil microcosms[J]. Applied and Environmental Microbiology, 1997, 63(2):679-686.
    [30] SALYERS A A, AMÁBILE-CUEVAS C F. Why are antibiotic resistance genes so resistant to elimination?[J]. Antimicrobial Agents & Chemotherapy, 1997, 41(11):2321-2325.
    [31] BAKER-AUSTIN C, WRIGHT M S, STEPANAUSKAS R, et al. Co-selection of antibiotic and metal resistance[J]. Trends in Microbiology, 2006, 14(4):176-182.
    [32] LI Y Z, CHEN H Y, SONG L T, et al. Effects on microbiomes and resistomes and the source-specific ecological risks of heavy metals in the sediments of an urban river[J]. Journal of Hazardous Materials, 2021, 409:124472.
    相似文献
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

谢庆超,李豪,栗瑞红,张红敏,刘海泉,潘迎捷,赵勇.牡蛎中细菌及其抗性基因多样性分析[J].上海海洋大学学报,2022,31(5):1260-1271.
XIE Qingchao, LI Hao, LI Ruihong, ZHANG Hongmin, LIU Haiquan, PAN Yingjie, ZHAO Yong. Diversity analysis of bacteria and their resistance genes in oyster[J]. Journal of Shanghai Ocean University,2022,31(5):1260-1271.

复制
分享
文章指标
  • 点击次数:1909
  • 下载次数: 1510
  • HTML阅读次数: 258
  • 引用次数: 0
历史
  • 收稿日期:2022-07-12
  • 最后修改日期:2022-08-29
  • 录用日期:2022-08-30
  • 在线发布日期: 2022-10-12
文章二维码