不同体色锦鲤和“福瑞鲤2号”mtDNA D-loop序列遗传变异分析

Genetic variation analysis of mtDNA D-loop sequences in different body color koi carp and FFRC No.2 strain common carp

摘要:

为探讨不同体色锦鲤和“福瑞鲤2号”遗传多样性及变异情况,基于mtDNA D-loop序列对6种颜色(全白、全红、红白、大正三色、白写、绯写)锦鲤、2个“福瑞鲤2号”(青灰和红色)及2个杂交鲤群体(青灰♀×红白锦鲤♂、红白锦鲤♀×青灰♂),共150尾个体进行序列分析。结果发现,全长932 bp的D-loop序列,A、T、G、C含量为33.2％、32.8％、14.2％和19.8％。所有个体共检测到34个变异位点,呈18个单倍型。其中,红白和绯写存在2个优势单倍型,全白和青灰“福瑞鲤2号”均存在2个特有单倍型,青灰、红色“福瑞鲤2号”共享1个优势单倍型。10个鲤群体间遗传距离为0.001～0.018,遗传分化指数(F_ST) 为0.011～0.933。分子方差(AMOVA)分析群体间变异67.17％,群体内变异32.83％,呈极显著分化水平。研究表明,绯写、全白和大正三色3个锦鲤群体具较高遗传多样性,白写、“福瑞鲤2号”(青灰和红色)及杂交鲤遗传纯度较高,结果丰富了不同体色鲤种质资源遗传数据,为后期种质资源利用和遗传选育提供参考。

Abstract:

In order to investigate the genetic diversity and variation of different body colors of common carp (Cyprinus carpio), a total of 150 individuals from six different colored koi carp populations (Platinum Ogon, Higoi, Kohaku,Tajsho-sanke, Shiro Utsuri and Hi Utsuri), two colored FFRC No.2 strain common carp (grey and red) populations, and two hybrid carp populations (grey FFRC No.2 strain common carp♀ × Kohaku koi♂,Kohaku koi♀ × grey FFRC No.2 strain common carp♂) were analyzed based on mtDNA D-loop sequences. The results showed that the contents of A, T, G and C in the 932 bp D-loop sequence were 33.2%, 32.8%, 14.2% and 19.8%, respectively. In total, 34 variant sites were detected in all individuals, showing 18 haplotypes. Among them, there were two dominant haplotypes in Kohaku and Hi Utsuri, respectively. There were two unique haplotypes in white koi carp and grey FFRC No.2 strain common carp, and grey and red FFRC No.2 strain common carp shared one dominant haplotype. The genetic distances across the ten populations ranged from 0.001 to 0.018, with F_ST values ranging from 0.011 to 0.933. The analysis of molecular variance (AMOVA) revealed that there was 67.17% variation among varieties and 32.83% variation within varieties, indicating highly substantial genetic difference. It demonstrated that the Hi Utsuri, white koi carp, and Tajsho-sanke had high genetic diversity, while the Shiro Utsuri, FFRC No.2 strain common carp (grey and red), and hybrid carp had high genetic purity. The study enriched the genetic data of the various body color carp germplasm resources and provided the guidance for germplasm resource utilization and genetic selection in the future.