一株新分离雨生红球藻培养条件的优化

Optimization of culture conditions for a newly isolated microalga Haematococcus pluvialis

摘要:

为探究一株新分离雨生红球藻的适宜生长条件,以藻细胞密度为响应值,通过单因子试验方法,获得该株雨生红球藻生长的最佳光照强度、培养温度和接种密度分别为105 μmol/(m²·s)、21 ℃和 7.5×10⁴ cells/mL。然后采用响应曲面法(RSM)耦合Box-Behnken设计,建立了预测雨生红球藻细胞密度的二次多项式方程,培养光照强度、温度和接种密度及其二项式对雨生红球藻细胞培养密度有着显著影响。各因素影响强弱的顺序:接种密度>光照强度>培养温度。响应曲面法获得雨生红球藻的最佳培养条件为光照强度120 μmol/(m²·s),温度20 ℃,接种密度9.25×10⁴ cells/mL,在此组合条件下雨生红球藻细胞密度可达45.08×10⁴ cells/mL,比单因素实验得到的最高藻细胞密度高出6.9%。

Abstract:

The suitable culture conditions for the growth of a newly isolated microalga Haematococcus pluvialis were investigated. The light intensity, culture temperature and inoculation density for optimal growth of the microalga were obtained as 105 μmol/(m²·s), 21 ℃and 7.5×10⁴ cells/mL respectively based on cell density as the indicator via single factor test. The growth performance (cell density) of the microalga could be predicted with the quadratic polynomial equation established by response surface method (RSM) coupled with Box -Behnken design. Light intensity, culture temperature and inoculation density as well as their binomial had significant effects on the cell density of H. pluvialis in cultivation. The weight of influence from the factors was as follows: inoculation density > light intensity > culture temperature. The combined culture conditions for optimal growth of H. pluvialis predicted by RSM were light intensity 120 μmol/(m²·s), temperature 20 ℃ and inoculation density 9.25×10⁴ cells/mL.Confirmatory test showed a cell density of 45.08 ×10⁴ cells/mL in culture was obtained under the predicted optimal combined culture conditions, which was 6.9% higher than the highest cell density of the culture under optimal culture conditions based on single factor test.