含虾青素的雨生红球藻粉自微乳的制备及体外评价

Preparation of Haematococcus pluvialis powder self-microemulsion containing astaxanthin and in vitro evaluation

摘要:

为了提高雨生红球藻粉中虾青素的溶出度，使用自乳化给药系统。采用伪三元相图法筛选液体自乳化给药系统的最佳组成，使用未经提取的雨生红球藻粉制备含虾青素的自微乳，并进行体外评价。以油相、乳化剂、助乳化剂用量为影响因素，平均粒径、多分散指数（PDI）、雨生红球藻粉中虾青素的载药量为评价指标，响应面法优化处方。然后，考察自微乳外观形态、粒径、PDI、Zeta电位、载药量、溶出度。最佳处方为油相（三乙酸甘油酯）用量5%，乳化剂（吐温80）用量67.86%，助乳化剂（聚乙二醇800）用量27.14%，在透射电镜下观察到微乳呈圆球状分布，大小均匀，平均粒径（50.32±0.33）nm，PDI（0.131±0.02），Zeta电位（-14.6±0.06）mV，虾青素载药量为（3.06±0.23）mg/g。含虾青素的雨生红球藻粉自微乳制备简单，增加了虾青素在水中的溶解度，有利于胃肠道吸收，体系具有良好的稳定性，在食品和药品工业中具有广阔的应用前景。

Abstract:

In order to improve the dissolution of astaxanthin from Haematococcus pluvialis powder, a self-emulsifying drug delivery system was used. The pseudo ternary phase diagram method was used to screen the best composition of the liquid self-emulsifying drug delivery system, and the unextracted Haematococcus pluvialis powder was used to prepare astaxanthin-containing self-microemulsion, and to conduct in vitro evaluation. Taking oil phase, emulsifier, and co-emulsifier dosage as influencing factors, average particle size, polydispersity index (PDI), and drug loading of astaxanthin in Haematococcus pluvialis powder as evaluation indicators, response surface methodology was used to optimize the prescription. Then, the appearance, particle size, PDI, Zeta potential, drug loading, and dissolution of the self-microemulsion were investigated. The best prescription is oil phase (glyceryl triacetate) dosage of 5%, emulsifier (Tween 80) dosage of 67.86%, and co-emulsifier (polyethylene glycol 800) dosage of 27.14%. Under the transmission electron microscope, it was observed that the microemulsion was distributed in a spherical shape with uniform size. The average particle size was (50.32±0.33) nm, PDI (0.113±0.02), the Zeta potential was(-14.6±0.06) mV, and the astaxanthin drug loading was (3.06±0.23) mg/g. The self-microemulsion of Haematococcus pluvialis powder containing astaxanthin was simple to prepare, which increased the solubility of astaxanthin in water, and was conducive to gastrointestinal absorption. The system has good stability and has broad application prospects in food and drug industry.