应用酸性电解水联合超声波杀灭副溶血性弧菌

Combination of acidic electrolyzed water with ultrasonic for killing Vibrio parahaemolyticus

摘要:

将酸性电解水与超声波技术相结合，探究其对副溶血性弧菌的杀灭情况，并与其他杀菌措施进行对比。采用平板计数法进行菌落计数，扫描电镜观察细菌的形态变化，蛋白质泄漏揭示细胞膜通透性差异，并结合流式细胞仪分析生物学特征的改变，分别比较了酸性电解水、超声波以及联合处理对副溶血性弧菌的杀菌效果。结果显示：酸性电解水联合超声波处理可使副溶血性弧菌的数量减少2.09 log CFU/mL，亚致死菌数量为1.80 log CFU/mL，而仅用超声波处理，细菌只减少了0.63 log CFU/mL，亚致死菌数量为0.05 log CFU/mL（P<0.05）。扫描电镜结果表明，电解水联合超声波处理对副溶血性弧菌的细胞结构有明显的破坏作用，结合二喹啉甲酸法（BCA）显示其细胞内蛋白质泄漏226.596 μg/mL（P<0.05）。进一步的流式细胞仪分析结果显示，经联合处理后细菌细胞明显缩小，颗粒度变化增大。综上所述，相较于酸性电解水或超声波单一处理，通过菌落计数，细菌的形态变化，蛋白质泄漏与细胞生物学特征的改变可知，酸性电解水联合超声波的处理方式具有更强的杀菌效果，可作为一种新型的技术应用于水产品中副溶血性弧菌的杀灭。

Abstract:

This study evaluated the bactericidal effects were evaluated against Vibrio parahaemolyticus by acidic electrolyzed water (AEW) combined with ultrasound which is a method different from the traditional sterilization methods. The effects were compared by viable plate count analysis. The physiological response was studied by scanning electronic microscopy (SEM) and cell membrane permeability revealed by protein leakage. Flow cytometry analysis revealed changes in the physiological states of V.parahaemolyticus. The effects of the combination of SAEW and ultrasound on the reductions of V.parahaemolyticus were evaluated in comparison with acidic electrolyzed water or ultrasound. The results revealed that AEW combined with ultrasonic showed bacterial reduction in V.parahaemolyticus by 2.09 log CFU/mL and the sub-lethal bacterial reduction was 1.80 log CFU/mL,when compared to the reduction of 0.63 log CFU/mL and the sub-lethal bacterial reduction of 0.05 log CFU/mL(P<0.05) after ultrasonic treatment only. Scanning electron microscopy (SEM) results showed that electrolyzed water combined with ultrasonic treatment had obvious destructive effect on the cell structure of V.parahaemolyticus. The protein leakage of V.parahaemolyticus detected by bicinchoninic acid (BCA) were 226.596 μg/mL, respectively, and difference was significant (P<0.05). Flow cytometry analysis showed that the cells became smaller and the change in particle size increased. In summary, acidic electrolyzed water combined with ultrasound could be more effective in reducing V.parahaemolyticus, the physiological response changes, protein leakage and changes in the physiological states, when compared with acidic electrolyzed water or ultrasound alone. The combination of acidic electrolyzed water and ultrasound could be an new technique to reduce the risk of V.parahaemolyticus.