金鱼类胡萝卜素提取工艺对提取效果的差异性

Differences in extraction efficiency of different extraction processes of goldfish carotenoids

摘要:

以红草金鱼（red Carassius auratus）为研究对象，探究不同提取工艺对其体内类胡萝卜素提取效果的差异性。研究分别通过单因素试验和正交试验，确定不同影响因素的最佳提取条件，结果表明提取样品为红草金鱼干样、提取料液比为1∶15、提取时间为90 min、提取次数为2次、V（乙醇）∶V（丙酮）=1∶2、提取温度为80℃，类胡萝卜素提取含量质量分数最高可达140.58 mg/kg。筛选最优提取工艺，为今后提取与测定金鱼体内类胡萝卜素含量及其各组分色素的研究提供重要的科学依据。

Abstract:

Red Carassius auratus was used to investigate differences in the extraction efficiency of carotenoids by different extraction processes. The optimum extraction conditions including different factors were determined by single factor test and orthogonal test respectively. According to the results, the best extraction conditions of carotenoids extraction process of red Carassius auratus are showed as follows:dried red Carassius auratus was used as the sample, the ratio of solid to liquid is 1:15, the extraction was conducted at 80℃, for 90 min each time, twice, organic solution ratio (ethanol to acetone) is 1:2. The carotenoid extracted can reach up to 140.58 mg/kg. An important basis is provided for the development on the extraction process afterwards and some references are also provided for the further study on the determination of carotenoids in goldfish.