船舶压载水与沉积物细菌多样性比较分析

Comparative analysis of bacterial diversity in ship ballast water and sediments

摘要:

利用Illumina HiSeq 2500高通量测序平台对船舶压载水及沉积物微生物进行测序，共获得67 255条有效序列，736个OTU，通过生物信息分析，研究了压载舱中沉积物和压载水细菌群落结构及其多样性。结果显示：船舶压载舱沉积物中可培细菌总数较压载水中可培细菌总数高3个数量级。压载水中优势类群是变形菌（Proteobacteria 70.20%）、拟杆菌（Bacteroidetes 13.38%）、放线菌（Actinobacteria 10.33%）和Marinimicrobia_SAR406_clade 2.81%。压载舱中沉积物的优势类群为变形菌（Proteobacteria 95.45%）、厚壁菌（Firmicutes 2.23%）。

Abstract:

Illumina HiSeq 2500 High-throughput Sequencing Platform was used to sequence the microorganisms samples collected from ship's ballast water and sediment. A total of 67 255 valid sequences and 736 OTUs were obtained. This study focuses on bacterial community structure and diversity of sediment and ballast water in ballast tanks by bioinformatics analysis.The results show that the total number of cultured bacteria of sediment is 3 orders of magnitude higher than the total number of cultured bacteria of ballast water.The dominant groups in the ballast water were Proteobacteria(70.20%),Bacteroidetes(13.38%),Actinobacteria(10.33%) and Marinimicrobia_SAR406_clade(2.81%). The dominant groups of sediments in ballast tanks were Proteobacteria (95.45%) and Firmicutes (2.23%). This study provides scientific basis for the research of ballast water ecology and prevention of biological invasion in ballast water.