罗氏沼虾5个专门化品系选择系繁殖力的比较分析

Comparative analysis of fecundity of 5 specialized strains of shrimp Macrobrachium rosenbergii

摘要:

为探讨罗氏沼虾繁殖性能，本实验通过测定5个罗氏沼虾专门化品系选择系（A、B、C、D、E 系）连续3次抱卵的单位体质量抱卵量（F_W）、卵巢发育周期（C_O）、受精卵卵径（D_E）、卵黄蛋白原（VTG）含量、组织蛋白酶D（CTSD）活性等指标，比较分析5个选择系的繁殖力，以期筛选出高繁殖力品系罗氏沼虾。结果显示：罗氏沼虾绝对抱卵量（F）、F_W与体质量正相关显著（P<0.05），F与F_W呈极显著正相关（P<0.01）；5个选择系的三次抱卵F_W平均值范围为（1.96~2.30）×10³粒/g，各系间差异显著（P<0.05），其中B和D的F_W较高，分别为2.26×10³粒/g和2.30×10³粒/g；5个选择系受精卵中VTG含量、CTSD活性的平均值范围分别为3.48~5.80 μg/g、271.81~320.82 U/g，各系间差异均显著（P<0.05），其中受精卵VTG含量最高为B的5.80 μg/g，CTSD活性最高为D的320.82 U/g，次之为B的303.09 U/g，两者无差异（P>0.05）；C_O范围为18.8~23.8 d，第二次大于第一次（2~8 d），各系间无差异（P>0.05），但均显著小于对照组（P<0.05）；D_E平均值范围为542.3~552.0 nm，各系间无差异，B、D选择系D_E均值较大，分别为551.7 nm、552.0 nm。因此可认为罗氏沼虾5个专门化品系选择系中B和D的繁殖力最强，A选择系次之，C和E选择系最弱，此可为罗氏沼虾专门化品系选择系的选育提供依据。

Abstract:

In order to investigate the breeding performance of Macrobrachium rosenbergii, this experiment was conducted by measuring 5 specialized strains of shrimp Macrobrachium rosenbergii (A, B, C, D, E) of the three consecutive spawning unit weight of fecundity (F_W) ovarian development cycle (C_O), fertilized egg diameter (D_E), vitellogenin (VTG) content, and cathepsin D (CTSD) activity index, and comparative analysis of 5 selection strains of fertility was made, with a view to screening the high fecundity strains of Macrobrachium rosenbergii. The results showed that the absolute fecundity (F), F_W and body weight were positively correlated (P<0.05), F and F_W were also significantly positively correlated (P<0.01). The average value of F_W of the continuous thrice spawning of the 5 selected strains was (1.96-2.30)×10³ eggs/g, the differences between the strains were significant (P<0.05).The F_W of B and D were 2.26×10³ eggs/g and 2.30×10³ eggs/g respectively. The average range of VTG content and CTSD activity in the 5 selected strains were 3.48-5.80 μg/g, 271.81-320.82 U/g, and there was significant difference among the strains (P<0.05),while the highest content of VTG in fertilized eggs was B (5.80 μg/g), the highest CTSD activity was D (320.82 U/g), the second was B (303.09 U/g), and there was no difference between them (P>0.05). The range of Co was 18.8-23.8 d, second time larger than the first (2-8 d), there was no difference (P>0.05) between each strain,which were all significantly lower than those in the control group (P<0.05). The mean value range of D_E was 542.3-552.0 nm, there is no difference among strains, and the average egg diameters of B and D were larger, 551.7 nm and 552.0 nm, respectively. It can be confirmed that among 5 specialized strains of Macrobrachium rosenbergii, the fecundity of B and D is the strongest, A is weak, C and E is the weakest, which can provide basis for breeding of specialized strains of shrimp Macrobrachium rosenbergii.