静水压法诱导双斑东方鲀三倍体的研究

Triploidy induction by hydrostatic pressure treatment in Takifugu bimaculatus

摘要:

为了更全面地研究双斑东方鲀三倍体诱导方法，促进双斑东方鲀产业健康发展，采用静水压法，探讨了诱导双斑东方鲀三倍体的起始时间、压强大小和处理时间长度等的最适诱导条件。实验结果表明：在水温18~20℃时，授精后5 min，采用10~15 Mpa的压强、5~7 min的处理时间的静水压诱导条件，可获得96.67%以上的三倍体诱导率，孵化率亦能达77%以上。本文首次建立了双斑东方鲀的三倍体苗种静水压诱导法，获得了高效的双斑东方鲀三倍体诱导流程，并为其他东方鲀类的生产性三倍体诱导研究提供了参考。

Abstract:

Comprehensive studies on triploid induction technology will greatly improve the development of Takifugu bimaculatus industry. This study applied hydrostatic pressure method to induce T. bimaculatus triploidy and investigated optimal inducing conditions of the starting time, the pressure and the treatment duration. Results showed that 96.67% triploid rate and 77% hatching rate were achieved under such condition:treatment temperature as 18-20℃, starting at 5 min after fertilization, hydrostatic pressure as 10-15 Mpa and treatment duration as 5-7 min. This is the first report for T. bimaculatus triploidy by hydrostatic pressure,which may provide an efficient technological process for T. bimaculatus triploidy. It also can offer a good reference for triploid induction in other Takifugu species.