水温及其波动对海膜生长和生理生化的影响

Effects of constant and fluctuating water temperature on growth and physiological and biochemical components of Halymenia sinesis

摘要:

研究了在不同水温（17、21、25、29、33 ℃）及不同变温幅度[（25±0）、（25±2）、（25±3）、（25±4）、（25±5） ℃]下海膜的生长及光合色素、POD、SOD、MDA随时间的动态变化。研究结果表明：（1）海膜在17～29 ℃范围内都能生长，在25 ℃以及±2 ℃变温下有最大的日相对生长率。当温度高于29 ℃时，藻体变软颜色变淡，高于33 ℃藻体局部变白溃烂。（2）海膜在低于25 ℃条件下生长，其POD、SOD和MDA值变化不明显；而29 ℃时，海膜的POD、SOD和MDA值随时间升高很快，33 ℃时海膜的POD、SOD和MDA值先升高，从第5天开始下降，可能是由于藻体受到胁迫较严重，发白溃烂，细胞受损。（3）与25 ℃恒温相比，±4和±5 ℃变温幅度下海膜的POD、SOD和MDA值整体呈上升趋势，且±5 ℃﹥±4 ℃，±2、±3 ℃变温幅度下海膜的POD、SOD和MDA值变化幅度不大，（25±2） ℃﹥（25±3） ℃﹥25 ℃，说明海膜对小范围的温度波动有一定适应性，变温幅度越大，对海膜的胁迫越大。

Abstract:

We studied the effects of different water temperature (17, 21, 25, 29, 33 ℃) and the fluctuating temperature (25±0, 25±2, 25±3, 25±4, 25±5 ℃) on the growth,pigment,POD,SOD and MDA of Halymenia sinesis. The results show:(1) the suitable temperature for H. sinesis growth was 17-29 ℃ with 25 ℃ and (±2, ±3 ℃) temperature fluctuation as the optimum growth temperature. When the temperature was above 29 ℃, the H. sinesis become soft and light color; above 33 ℃, the H. sinesis rotted locally.(2) The POD, SOD and MDA were stable when the temperature was below 25 ℃ and above 17 ℃.But they increased soon at 29 ℃or rise at first and decline from the fifth day at 33 ℃,which indicates that the H. sinesis suffered serious stress, so its cells damaged and decayed.(3) Compared with 25 ℃, the POD, SOD and MDA changes are stable in (±2, ±3 ℃) temperature range, and (25±3 ℃)﹥(25±2 ℃)﹥25 ℃.In addition, the change is severe in (±4, ±5 ℃) temperature range, and(25±5 ℃)﹥(25±4 ℃). To sum up, the H. sinesis can adapt to small temperature fluctuation. The greater the temperature difference, the greater the damage to H. sinesis.