急性操作胁迫对美洲鲥亲鱼血清生化指标及HSP70基因表达的影响

Effects of acute handling stress on serum biochemical parameters and HSP70 gene expression in Alosa sapidissima broodstocks

摘要:

探讨了急性操作胁迫对美洲鲥（Alosa sapidissima）亲鱼血清生化指标及鳃和肝组织HSP70（Heat Shock Protein70） mRNA水平的影响。采用空气暴露方式对美洲鲥亲鱼进行了急性操作胁迫。实验设定应激零点对照组和15 s、30 s、60 s 3个操作强度实验组，操作后采样，测定皮质醇（COR）、三碘甲腺原氨酸（T3）、甲状腺素（T4）、血清血糖（GLU）、甘油三酯（TG）、总胆固醇（TC）、总蛋白（TP）、谷丙转氨酶（ALT）、谷草转氨酶（AST）、谷胱甘肽S转移酶（GST）、溶菌酶（LZM）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、丙二醛（MDA）、Na⁺、K⁺、Cl^-、Ca²⁺以及鳃、肝HSP70 mRNA水平。结果表明：与对照组相比，实验组COR、ALT、AST、GST、LZM、SOD、MDA、GLU、TG、TC、TP、K⁺和Ca²⁺均有显著增加（P<0.05）；CAT和T4出现显著下降（P<0.05）；T3、Na⁺和Cl^-未见显著差异（P>0.05）。血清生化指标显著变化表明，急性操作胁迫下美洲鲥亲鱼的肝脏、心脏损伤指标显著升高，表明肝脏以及心脏可能受到了一定程度损伤。与对照组相比，实验组鳃HSP70 mRNA水平未有显著变化（P>0.05），肝HSP70 mRNA水平在15 s、30 s和60 s空气暴露组均显著升高（P<0.05）。表明急性操作胁迫可以提高肝脏HSP70 mRNA水平。

Abstract:

This experiment studied the effect of acute handling stress on serum biochemical parameters and HSP70mRNA level in gill and liver of Alosa sapidissima broodstocks. The air exposure was applied in the acute handling stress of Alosa sapidissima broodstocks. This experiment set one stressless control group and three different intensity stress groups, separately exposed air 15 s, 30 s and 60 s. Serum cortisol(COR), triiodothyronine(T3), thyroxine(T4), glucose(GLU), triglyceride (TG), total cholesterol (TC), total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione S-transferase (GST), lysozyme (LZM), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), Na⁺, K⁺, Cl^-, Ca²⁺ concentrations and gill, liver HSP70 mRNA level were determined after sampling. The results showed that compared with the control group, the treatment group had the following serum biochemical parameters significantly increased: COR, ALT, AST, GST, LZM, SOD, MDA, GLU, TG, TC, TP, K⁺ and Ca²⁺ (P<0.05). CAT and T4 decreased significantly(P<0.05) after air exposure, while T3, Na⁺ and Cl^- had no significant difference(P>0.05). The changes in serum biochemical parameters indicate that the liver and heart of the Alosa sapidissima damage index significantly increased, suggesting that liver and heart may have been damaged to a certain extent. Additionally, compared with the control group, there was no significant change in gill HSP70 mRNA level in the experimental group (P>0.05). Liver HSP70 mRNA level in 15s, 30s and 60s were significantly higher in the exposed group (P<0.05). Acute handling stress can improve the level of HSP70 mRNA in liver.