牙鲆仔鱼SWS1基因克隆及5个视蛋白基因的表达

Cloning of SWS1 gene and expression of five opsin genes in larval flounder Paralichthys olivaceus

摘要:

鱼类主要通过视蛋白来感知光强和波长。本研究首次克隆到牙鲆(Paralichthys olivaceus)短波紫外敏感视蛋白SWS1基因全长cDNA序列共1 317 bp,包括含1 077 bp的开放阅读框,编码蛋白358个氨基酸,表明牙鲆仔鱼具有潜在感知紫外线的能力。此外,qRT-PCR显示视杆视蛋白RH1和SWS1基因表达无显著性变化;而中波敏感视蛋白RH2和短波蓝色敏感视蛋白SWS2基因表达从孵化后第1天到第13天显著性增强;长波敏感视蛋白LWS基因从孵化后第1天到第6天显著性增强,而此后到第13天显著性降低。RNA整体原位杂交显示,眼睛中感蓝光的SWS2基因表达较其他视蛋白基因晚。视蛋白基因表达信号主要分布在视网膜及晶体周围,此外还首次发现这些视蛋白基因在皮肤、鳍条、鳃和肠道等组织中表达。

Abstract:

Fish perceive light intensity and wavelength mainly through visual opsins. The 1 317 bp full-length SWS1 cDNA sequence of Paralichthys olivaceuswas first cloned in this research containing 1 077 bp length ORF, encoding 358 amino acid of cone opsin. It indicated that the larval flounder have the potential ability to perceive ultraviolet rays. In addition, qRT-PCR showed that the related expression of rod opsin RH1 and SWS1 had no significant changes. While the related expression of cone opsin genes RH2 and SWS2 was significantly enhanced from first hatched larvae to 13th day after hatching(DAH) larvae; LWS gene was significantly enhanced from DAH6, and then descend to DAH13 slowly. In eyes, furthermore, RNA whole mount in situ hybridization revealed that the signals of opsin genes were mainly expressed in the peripheral retina and crystal and SWS2 expressed later than other opsin genes. Unexpectedly, the expressions of opsin genes were found in skin, fins, gills, intestine and other tissues extensively.