中华绒螯蟹Myostatin基因在蜕皮过程中的表达分析

Expression analysis of Myostatin during molting cycle in Eriocheir sinensis

摘要:

通过荧光定量PCR(qRT-PCR)技术对中华绒螯蟹(Eriocheir sinensis)Myostatin(EsMSTN)基因在不同组织及蜕皮阶段的表达情况进行了分析。结果显示:(1) EsMSTN在蜕皮间期(C期)中华绒螯蟹肌肉组织中的基因表达水平最高,胸神经节和脑神经节中也有较高水平的表达,暗示EsMSTN基因的功能并不局限于肌肉生长的调节。(2) 在不同蜕皮阶段,肌肉中EsMSTN的基因表达水平在蜕皮后期(AB期)最高,蜕皮前期(D期)最低,推测EsMSTN可能正向调节中华绒螯蟹肌肉生长,参与肌肉组织蜕皮后的生长以及蜕皮前的萎缩过程;肝胰腺、上皮组织中EsMSTN的最高基因表达水平都出现蜕皮后的AB期,C期或D期相对表达量最低,这可能与上述器官的细胞增殖主要发生在AB期有关;胸神经节中EsMSTN的表达水平在蜕皮期(E期)最高,D期最低,暗示EsMSTN正向调节胸神经节中的神经发生。

Abstract:

In present study, the expression differences of myostatin(MSTN) in various tissues of Eriocheir sinensis during molting cycle were analyzed by qRT-PCR. The results showed that,(1) EsMSTN(Eriocheir sinensis MSTN) had its highest expression level in muscle, while higher expression levels were also observed in thoracic ganglia and brain ganglia at stage C, suggesting its physiological function was not restricted to muscle.(2) EsMSTN was expressed the highest at AB and the lowest at D stage of molting cycle in muscle, suggesting that EsMSTN might be a positive growth regulator in E. sinensis, involved in the significant expansion and severe atrophy of the muscle at AB stage and D stage, respectively. Whereas in hepatopancreas and epithelium, EsMSTN mRNA expression levels were the highest at AB stage, and the lowest at C or D stage, due probably to cell proliferation at AB stage in these tissues. In addition, the peak value of EsMSTN mRNA in thoracic ganglia occurred at stage AB and its lowest expression level was observed at stage D, which implied that EsMSTN may regulate the nerogenesis in this tissue positively.