坛紫菜与Pyropia radi的种间杂交实验
CSTR:
作者:
作者单位:

上海海洋大学,上海海洋大学,上海海洋大学

基金项目:

国家高技术研究发展计划(2012AA100811);国家自然科学基金(31072208);农业部公益性专项(200903030);国家海洋局公益专项(201105023,201105008);国家农业科技成果转化资金项目(2011GB2C000005);上海市科学技术委员会重点科技攻关项目(10391901100)


Study on the interspecies cross between Pyropia haitanensis and Pyropia radi
Affiliation:

College of Fisheries and Life Science,Shanghai Ocean University,College of Fisheries and Life Science,Shanghai Ocean University,College of Fisheries and Life Science,Shanghai Ocean University

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [26]
  • |
  • 相似文献 [20]
  • |
  • 引证文献
  • | |
  • 文章评论
    摘要:

    为探讨坛紫菜(Pyropia haitanensis)种间杂交的可行性,进一步选育坛紫菜新品系,以野生型坛紫菜作为母本,以采自印度的Pyropia radi作为父本进行种间杂交,并以亲本叶状体细胞的液泡大小与色素体的大小和颜色作为遗传标记:母本的细胞内液泡较小,具一个较大的色素体,颜色偏红;父本的细胞内具有一个很大的液泡,但色素体较小,颜色偏褐带黄。杂交实验结果如下:依据液泡体积、色素体的大小和颜色区分,在F1叶状体中出现了6种类型的细胞:2种亲本细胞类型,4种杂交重组细胞类型,它们分别为母本型(Ph),父本型(Pr),似母本型(Ph′,液泡大小与Ph相同,但色素体偏黄),似父本Ⅰ型(Pr′,液泡比Pr小,且色素体偏红),似父本Ⅱ型(Pr″,液泡大小介于Pr和Pr′之间,且色素体颜色比Pr′更浅),似父本Ⅲ型(Pr,液泡大小与Pr相同,但色素体稍黄)。这6种细胞在F1叶状体上出现分离,并呈块状直线排列。在F1叶状体中出现大量由2~4个色块构成的颜色嵌合体和少数单色的叶状体。上述结果证实这两种紫菜的种间杂交是成功的,并且F1叶状体是成活和可育的,为今后选育种间杂交新品种奠定了基础。

    Abstract:

    In order to verify the practicability of interspecies cross between Pyropia haitanensis and other Pyropia, cross experiment between P. haitanensis from the temperate water of China and P. radi from the tropical water of India was carried out in the laboratory, marked with the color and size of the vacuoles and the chloroplasts in cells of parental blades. P. haitanensis was used as female parent, and its blades show brown green with an obvious pigment in cells of blades. P. radi was used as male parent, and its blades show brown in color and the cells of blades containing a large vacuole and small chloroplasts. Among the F1 gametophytic blades from the cross of P. haitanensis (♀) × P. radi (), two parental colors (Ph and Pr) and four new colors (Ph′, the size of the vacuole similar to Ph and the color of the chloroplasts slightly yellower than Ph; Pr′, the size of the vacuole smaller than Pr and the color of the chloroplasts redder than Pr; Pr″, the size of the vacuole between Pr and Pr′ and the color of the chloroplasts lighter than Pr′; Pr, the size of the vacuole similar to Pr and the color of the chloroplasts slightly yellower than Pr) appeared. Linear segregation of colors occurred in the F1 blades, and therefore, the color sectored blades with 2-4 sectors were formed. There were a large number of color sectored blades and a few of single color blades. These results indicate that the interspecies cross between P. haitanensis and P. radi succeeded and the F1 gametophytic blades could survive and be fertile,which laid the foundation for the further selection of the new strains.

    参考文献
    [1] 陈昌生, 王凤霞, 徐燕, 等. 野生型与红色型坛紫菜杂交嵌合体及选育的研究 [J]. 海洋学报, 2007, 29(6): 101-109.
    [2] 李西林. 论坛紫菜壳孢子采苗时间 [J]. 福建水产, 1992, 2: 40-42.
    [3] 严兴洪, 马少玉. 坛紫菜抗高温品系的筛选 [J]. 水产学报, 2007, 31(1) :112-119.
    [4] 吕峰, 严兴洪, 刘长军, 等. 坛紫菜耐高温品系的选育与海区中试 [J]. 上海海洋大学学报, 2010, 19(4): 457-462.
    [5] 付春辉, 严兴洪, 黄林彬, 等. 条斑紫菜(Porphyra yezoensis)选育品系壳孢子的放散量与耐高温性研究 [J]. 海洋与湖沼, 2011, 42(3): 460-466.
    [6] 王华芝, 严兴洪, 李琳. 条斑紫菜(Porphyra yezoensis)耐高温品系的筛选及特性分析 [J].海洋与湖沼, 2012, 43(2): 363-369.
    [7]何培民, 秦松, 严晓军, 等. 海藻生物技术及其应用. 北京: 化学工业出版社, 2007, 86-98.
    [8] COMINGS D E, MACMURRAY J P. Molecular heterosis: a review [J]. Molecular Genetics and Metabolism, 2000, 71(1): 19-31.
    [9] 陈昌生, 徐燕, 纪德华, 等. 坛紫菜品系间杂交藻体选育及经济性状的初步研究 [J]. 水产学报, 2007, 31(1): 97-104.
    [10] 陈昌生, 纪德华, 谢潮添, 等. 坛紫菜耐高温品系选育及经济性状的初步研究 [J]. 海洋学报, 2008, 30(5): 100-106.
    [11] 徐燕, 谢潮添, 纪德华, 等. 坛紫菜品系间杂交分离色素突变体及其特性的初步研究 [J]. 中国水产科学, 2007, 14(3): 466-472.
    [12] 徐燕, 陈昌生, 谢潮添, 等. 坛紫菜杂交品系优势的初步评价 [J]. 海洋水产研究, 2008, 29(1): 62-69.
    [13] 纪德华, 谢潮添, 徐燕, 等. 坛紫菜品系间杂交子代杂种优势的ISSR分析 [J]. 海洋学报, 2008, 30(6): 147-153.
    [14] KIM N G. Culture study on the hybrid by interspecific crossing between Porphyra pseudolinearis and P. dentata (Bangiales, Rhodophyta), two dioecious species in culture [J]. Algae, 2011, 26(1): 79-86.
    [15] 严兴洪, 李琳, 陈俊华, 等. 坛紫菜的单性生殖与遗传纯系分离 [J]. 高技术通讯, 2007, 17(2): 205-210.
    [16] 粱志强. 坛紫菜遗传育种的初步研究 [D]. 上海: 上海水产大学硕士学位论文, 上海, 2004.
    [17] 王素娟, 张小平, 徐志东, 等. 坛紫菜营养细胞和原生质体培养的研究Ⅰ [J]. 海洋与湖沼, 1986, 17(3): 217-221.
    [18] YAN X H, ARUGA Y. Induction of pigmentation mutants by treatment of monospore germlings with with MNNG in Porphyra yezoensis Ueda (Bangiales, Rhodophyta) [J]. Algae, 1997, 12(3): 39-52.
    [19] 高洪峰. 不同生长期坛紫菜中藻胆蛋白的含量变化 [J]. 海洋与湖沼, 1993, 24(6): 645-648.
    [20] 严兴洪, 刘旭升. 坛紫菜雌雄叶状体的细胞分化比较 [J]. 水产学报, 2007, 31(2): 184-192.
    [21] NIWA K, MIZUTA A, ARUGA Y. Genetic characterization of a spontaneous green-type pigmentation mutant of Porphyra yezoensis and the significance of using heterozygous conchocelis in nori farming [J]. Fisheries Science, 2002, 68(4): 729-735.
    [22] 严兴洪, 田中次郎, 有贺佑胜. 条斑紫菜色彩突变体的诱导、分离和特性分析 [J]. 水产学报, 2000, 24(3): 221-228.
    [23] 严兴洪, 李 琳, 有贺佑胜. 坛紫菜减数分裂位置的杂交试验分析 [J]. 水产学报, 2006, 30(1): 1-8.
    [24] 崔彬彬, 李云. 高等植物细胞质遗传的研究进展 [J]. 河北林果研究, 2006, 21(1): 24-29.
    [25] 胡迎春. 传统忍冬科非孟德尔遗传方式及被子植物细胞质遗传起源与进化的究 [D]. 北京: 北京大学博士学位论文, 北京, 2010.
    [26] MITMAN G G, MEER J P V. Meiosis, blade development, and sex determination in Porphyra purpurea (Rhodophyta) [J]. Journal of Phycology, 1994, 30: 147-159.
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

刘海洋,李 琳,严兴洪.坛紫菜与Pyropia radi的种间杂交实验[J].上海海洋大学学报,2013,22(6):882-887.
LIU Hai-yang, LI Lin, YAN Xing-hong. Study on the interspecies cross between Pyropia haitanensis and Pyropia radi[J]. Journal of Shanghai Ocean University,2013,22(6):882-887.

复制
分享
文章指标
  • 点击次数:3959
  • 下载次数: 2873
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2013-04-17
  • 最后修改日期:2013-06-03
  • 录用日期:2013-09-18
  • 在线发布日期: 2013-11-07
  • 出版日期: 2013-11-15
文章二维码