泥蚶与牡蛎净化工艺优化初探

Preliminary studies of shellfishes(Tegillarca granosa and Ostrea plicatula) purification technique

摘要:

应用臭氧与二氧化氯对活体泥蚶和牡蛎进行微生物净化处理。首先对泥蚶和牡蛎进行暂养吐泥，每5 h换水一次，收集排泄物经离心计算相对吐泥量；臭氧净化处理浓度分别为0.4、0.8、1.6 mg/L，二氧化氯浓度分别为5、10、20 mg/L。结果表明：经5 h暂养吐泥即基本完成；用臭氧浓度0.4～1.6 mg/L或二氧化氯浓度5～20 mg/L净化处理24 h，细菌总数减少90%左右，大肠菌群与致病菌则由净化前的超标变成净化后的全面达标；净化处理后的泥蚶与牡蛎在5 ℃的条件下保存，10 d以内存活率为100%，净化处理后的泥蚶保存10 d再进行微生物检测依然达标。得出简便可行的净化工艺流程为：暂养吐泥5 h，再用0.4 mg/L臭氧净化24 h，然后用尼龙袋密封在5 ℃条件下保存。

Abstract:

Tegillarca granosa and Ostrea plicatula were treated with ozone and chlorine dioxide for purification technique research. The water was changed each five hours during temporary cultivation, and the relative mud spit quantity was weighed after centrifugation. The ozone concentration were 0.4 mg／L, 0.8 mg／L or 1.6 mg／L, respectively ; and the chlorine dioxide concentration were 5 mg／L，10 mg／L or 20 mg／L, respectively. The results show that five hours is enough for mud spit; the total amount of bacteria was reduced to 10%, when shellfish were dipped in 0.4-1.6 mg／L ozone or 5-20mg/L chlorine dioxide for 24 hours，the number of coliform and pathogenic bacteria was brought down from exceeding the standard (GB 18406.4—2001). Packed in plastic bags and stored in refrigerator(5 ℃) , Tegillarca granosa and Ostrea plicatula were still keeping 100% living rate for 10 days. Tegillarca granosa after being purified then frozen was stored at 5 ℃ for 12 days and its microorganism number still met the standard.The concise purification procedure is: temporary cultivation for five hours, then purified with 0.4 mg/L ozone for 24 hours, at last stored in sealed nylon at 5 ℃.