白斑红点鲑精子形态和超微结构的研究

Morphosis and ultrastructure of the spermatozoon of Salvelinus leucomaenis

摘要:

应用扫描和透射电镜观察了白斑红点鲑（Salvelinus leucomaenis）精子的形态和超微结构。白斑红点鲑精子由头部、中段和尾部组成。头部呈椭球形，主要结构是细胞核，核前端无顶体，后端有植入窝，植入窝较深，凹入细胞核的1／3，核中染色质致密，存在着不规则的网络状间隙；中段包括中心粒复合体和袖套，中心粒复合体主要由近端中心粒和远端中心粒组成，近端中心粒为典型的9组三联微管结构，与远端中心粒相互垂直，远端中心粒向下延伸形成轴丝。袖套与细胞核后端相连，含有丰富的线粒体和囊泡，部分线粒体彼此融合，形成复合线粒体，囊泡有两种；白斑红点鲑精子尾部细长，主要结构是轴丝，为典型的“9+2”微管结构，轴丝被许多囊泡包围，质膜向外突出形成上下对称，但大小不同的两个侧鳍。

Abstract:

The morphous and ultrastructural organization of Salvelinus leucomaenis spermatozoon was observed by scanning and transmission electron microscopes. The spermatozoon with no acrosome consisted of the head, middle piece and the tail. The head of the spermatozoon was oval shape and mainly consisted of the nucleus. The nucleus, within which there were some netlike gaps, consisted of electron dense chromatin materials. In posterior extremity of the nucleus, implantation fossa trapped into the internal to the 1/3. The middle piece consisted of centriolar complex and sleeve. The proximal centriole, which is made up of nine triplets of peripheral microtubules, is perpendicular to the distal centriol which extended downward to form the kinetosome of axial filament. The sleeve, which contained a high number of mitochondria and two kinds of vesicles, was connected to the posterior end of the nucleus. Some mitochondria were fused with each other and formed a complex chondriosome. The tail of 〖WTBX〗Salvelinus leucomaenis〖WTBZ〗 spermatozoon was slim and long. The main composition of the tail was axoneme whose structure was the typical model of “9+2” and surrounded by vesicles. The plasma membrane was prominent symmetrically up and down to form two lateral fins with different sizes.