冬季不同海区锯缘青蟹细胞膜脂肪酸组成比较

Comparison of fatty acid composition in cell membrane of Scylla Paramamosain between different waters in winter

摘要:

通过比较冬季不同海区锯缘青蟹（Scylla paramamosain）细胞膜脂肪酸组成，旨在理解冬季低温对甲壳动物细胞膜脂肪酸组成的影响。利用气相色谱法测定采自厦门和宁海海区锯缘青蟹鳃、肝胰腺和肌肉细胞膜中脂肪酸组成。结果显示：锯缘青蟹鳃和肝胰腺细胞膜中C16∶0和C16∶1在宁海海区显著低于厦门海区(P<0.01或P<0.05)；肝胰腺细胞膜中C18∶0在宁海海区显著低于厦门海区(P<0.01)；C20∶4在鳃、肝胰腺和肌肉细胞膜中宁海海区均显著高于厦门海区(P<0.01或P<0.05)；肝胰腺中C20∶5宁海海区也显著高于厦门海区(P<0.01)。鳃和肝胰腺细胞膜中饱和指数∑SFA/∑UFA宁海海区显著低于厦门海区(P<0.01或P<0.05)；肌肉细胞膜中无显著差异(P>0.05)。结果表明：冬季温度较低的宁海海区，细胞膜脂肪酸组成中不饱和脂肪酸增加，饱和指数下降，从而维持细胞膜的流动性和正常生理功能的执行。

Abstract:

Gas chromatograph method was used to measure fatty acid composition in cell membrane of mud crab Scylla paramamosain from the different waters in winter in order to understand the impact of low temperature on fatty acid composition in membrane and the adaptation in winter. The results showed that the different changes of fatty acid composition appeared in cell membranes of different organs and tissues in S. paramamosain C16∶0 and C16∶1 relative content in cell membranes of gills and hepatopancreas in Ninghai waters were significant lower than these in Xiamen waters respectively (P<0.01 or P<0.05); C18∶0 relative content in cell membrane of hepatopancreas in Ninghai waters was significantly lower than that in Xiamen waters (P<0.01); C20∶4 in cell membranes of gill, hepatopancreas and muscle in Ninghai waters increased in contrast with Xiamen waters (P<0.01 or P<0.05); C20∶5 in hepatopancreas cell membrane was also significantly higher than that in Xiamen waters (P<0.01). Saturation index of ∑SFA/∑UFA in cell membranes of gill and hepatopancreas in Ninghai waters significantly decreased compared with the Xiamen waters (P<0.01 or P<0.05). For saturation index in cell membrane of muscle, there was no significant difference between Ninghai and Xiamen waters (P>0.05). In conclusion, in Ninghai waters with the lower temperature in winter, the increase of relative contents of nonsaturation fatty acids in cell membrane results in the decrease of saturation index to be used to keep the fluidity of cell membrane and ensure its ability to perform biological function.