响应面法优化鳗鱼骨可溶性钙提取的研究

Study on extraction of soluble calcium from eel bone with response surface methodology

摘要:

试验采用响应面法分析研究不同量盐酸浓度、提取温度和反应时间对可溶性钙提取的影响，以探讨盐酸对鳗鱼骨可溶性钙提取的效果。结果表明，最佳提取工艺条件为：盐酸浓度3.06 mol/L，提取温度45.90 ℃，反应时间1.9 h。通过中心组合试验设计建立回归模型，失拟项不显著，该回归模型拟合程度好。经试验验证，钙提取率为22.75%，与理论预测值（22.60%）接近。显著性分析可见：盐酸浓度对钙提取率影响不显著；提取温度对钙提取率影响显著；反应时间对钙提取率影响极其显著。响应面法分析：盐酸浓度和提取温度交互作用对钙提取率影响不显著；盐酸浓度和反应时间交互作用对钙提取率影响显著；提取温度和反应时间交互作用对钙提取率影响极其显著。经原子吸收分光光度法分析，鳗鱼骨粉总钙含量为27.3%，表明钙溶出率为83.33%。

Abstract:

Response surface methodology was used to study the effect of different levels of concentration of hydrochloric acid, temperature and time on the extraction rate of calcium. Results showed that the optimal processing conditions were: the concentration of hydrochloric acid was 3.06 mol/L, the temperature was 45.90 ℃,the time was 1.9 h. Central composition design was used to build regression equation. The lack of Fit not significant showed the good fit of the regression model. Experiments showed that the extraction rate was 22.75% under this condition consistent with the theoretical prediction 22.60%. Response surface methodology analysis: interaction of concentration of hydrochloric acid and temperature on the yield of calcium was not significant; interaction of concentration of hydrochloric acid and time on the yield of calcium was significant; interaction of temperature and time on the yield of calcium was significant. Through the analysis of atomic absorption spectrophotometry, total calcium of the eel bone meal was 27.3%. The dissoluble rate of calcium was 83.33%.