3种来源的野生斑节对虾携带病毒情况调查

Investigation on status of carrying virus of wild Penaeus monodon from three resources

摘要:

利用PCR方法，对2008年取自3个斑节对虾主产地（中国、泰国、非洲）的野生斑节对虾亲虾进行主要病毒病筛查。在所检测的210例野生亲虾样本中，均发现携带白斑综合征病毒（WSSV）、传染性皮下及造血组织坏死病毒（IHHNV）或斑节对虾杆状病毒（MBV）的阳性样本，其中泰国来源的斑节对虾亲虾WSSV携带率最高，达到89.8%，而中国来源的斑节对虾亲虾IHHNV携带率最高，为8.8%。12例样本同时检测出WSSV和IHHNV（阳性率为5.7%）。该结果揭示3种来源的斑节对虾亲虾普遍携带WSSV和IHHNV，其中WSSV仍然是感染斑节对虾的主要病原。调查结果增进了对斑节对虾主产区野生亲虾健康状况的了解，并在一定程度上揭示了WSSV感染的新规律。

Abstract:

In our research, we used PCR technology to examine the viral pathogen of wild Penaeus monodon from three main producing areas, including China, Thailand, and Africa in 2008. In the 210 wild Penaeus monodon broodstocks, we detected the positive result that some of them were carrying white spot syndrome virus (WSSV), infectious hypodermal and hematopoietic necrosis virus (IHHNV) or Monodon baculovirus (MBV). The results showed that the penaeid shrimps from Thailand had a highest WSSVcarrying rate of 89.8%, and those from China had a highest IHHNVcarrying rate of 8.8%. Twelve samples carrying WSSV and IHHNV (positive rate is 5.7%) were detected. These results reveal the broodstocks from China, Thailand and Africa are ubiquitously infected by WSSV and IHHNV. Meanwhile, WSSV is still a major pathogen of penaeid shrimp. Our research enhanced the understanding of the healthy condition of wild〖WTBX〗 Penaeus monodon〖WTBZ〗 broodstock from the three main producing areas, and also revealed the new pattern of WSSV infection to a certain degree.